The ail Gene Is Present in Some Yersinia enterocolitica Biotype 1A Strains

被引:38
作者
Sihvonen, Leila M. [1 ]
Hallanvuo, Saija [2 ]
Haukka, Kaisa [1 ]
Skurnik, Mikael [3 ,4 ]
Siitonen, Anja [1 ]
机构
[1] Natl Inst Hlth & Welf THL, Bacteriol Unit, FI-00271 Helsinki, Finland
[2] Finnish Food Safety Author Evira, Helsinki, Finland
[3] Univ Helsinki, Dept Bacteriol & Immunol, Haartman Inst, Helsinki, Finland
[4] Univ Helsinki, Cent Hosp, Diagnost Lab, Helsinki, Finland
关键词
POLYMERASE-CHAIN-REACTION; VIRULENCE GENES; PCR METHOD; FOOD;
D O I
10.1089/fpd.2010.0747
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
One chromosomal virulence marker of Yersinia is the gene ail, which encodes Ail, an outer membrane protein that promotes attachment and invasion. A high correlation has been found between the ail gene and the virulence of Yersinia. Here, we report two Yersinia enterocolitica biotype 1A strains that are usually nonpathogenic and carry the ail gene. The ail gene sequences of biotype 1A strains displayed similarity to the bioserotype 1B/O:8 strain 8081. The finding suggests that ail-based detection methods for Y. enterocolitica alone are insufficient to detect real pathogenic strains.
引用
收藏
页码:455 / 457
页数:3
相关论文
共 12 条
[1]   BACTERIAL-RESISTANCE TO COMPLEMENT KILLING MEDIATED BY THE AIL PROTEIN OF YERSINIA-ENTEROCOLITICA [J].
BLISKA, JB ;
FALKOW, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (08) :3561-3565
[2]   The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes [J].
Cheyne, Bo M. ;
Van Dyke, Michele I. ;
Anderson, William B. ;
Huck, Peter M. .
JOURNAL OF WATER AND HEALTH, 2010, 8 (03) :487-499
[3]   Molecular typing and virulence markers of Yersinia enterocolitica strains from human, animal and food origins isolated between 1968 and 2000 in Brazil [J].
Falcao, Juliana P. ;
Falcao, Deise P. ;
Pitondo-Silva, Andre ;
Malaspina, Ana Carolina ;
Brocchi, Marcelo .
JOURNAL OF MEDICAL MICROBIOLOGY, 2006, 55 (11) :1539-1548
[4]   USE OF THE POLYMERASE CHAIN-REACTION AND DNA-SEQUENCING FOR DETECTION OF BARTONELLA-QUINTANA IN THE AORTIC-VALVE OF A PATIENT WITH CULTURE-NEGATIVE INFECTIVE ENDOCARDITIS [J].
JALAVA, J ;
KOTILAINEN, P ;
NIKKARI, S ;
SKURNIK, M ;
VANTTINEN, E ;
LEHTONEN, OP ;
EEROLA, E ;
TOIVANEN, P .
CLINICAL INFECTIOUS DISEASES, 1995, 21 (04) :891-896
[5]   Diagnosis of meningococcal meningitis by broad-range bacterial PCR with cerebrospinal fluid [J].
Kotilainen, P ;
Jalava, J ;
Meurman, O ;
Lehtonen, OP ;
Rintala, E ;
Seppälä, OP ;
Eerola, E ;
Nikkari, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (08) :2205-2209
[6]   Real-time PCR method for detection of pathogenic Yersinia enterocolitica in food [J].
Lambertz, S. Thisted ;
Nilsson, C. ;
Hallanvuo, S. ;
Lindblad, M. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (19) :6060-6067
[7]   A comparison between a PCR method and a conventional culture method for detecting pathogenic Yersinia enterocolitica in food [J].
Lambertz, ST ;
BallagiPordany, A ;
Nilsson, A ;
Norberg, P ;
DanielssonTham, ML .
JOURNAL OF APPLIED BACTERIOLOGY, 1996, 81 (03) :303-308
[8]   THE AIL LOCUS IS FOUND UNIQUELY IN YERSINIA-ENTEROCOLITICA SEROTYPES COMMONLY ASSOCIATED WITH DISEASE [J].
MILLER, VL ;
FARMER, JJ ;
HILL, WE ;
FALKOW, S .
INFECTION AND IMMUNITY, 1989, 57 (01) :121-131
[9]   Yersinia enterocolitica and Y-enterocolitica-like species in clinical stool specimens of humans: identification and prevalence of bio/serotypes in Finland [J].
Sihvonen, L. M. ;
Haukka, K. ;
Kuusi, M. ;
Virtanen, M. J. ;
Siitonen, A. .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2009, 28 (07) :757-765
[10]   PCR detection of virulence genes in Yersinia enterocolitica and Yersinia pseudotuberculosis and investigation of virulence gene distribution [J].
Thoerner, P ;
Bin Kingombe, CI ;
Bögli-Stuber, K ;
Bissig-Choisat, B ;
Wassenaar, TM ;
Frey, J ;
Jemmi, T .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (03) :1810-1816