Effects of experimental parameters on the signal intensity of capillary electrophoresis electrospray ionization mass spectrometry in protein analysis

被引：19

作者：

Liang, Z ^{[1
]}

Yang, Q ^{[1
]}

Zhang, WB ^{[1
]}

Zhang, LH ^{[1
]}

Zhang, YK ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

来源：

CHROMATOGRAPHIA | 2003年 / 57卷 / 9-10期

关键词：

capillary electrophoresis; electrospray ionization-mass spectrometry; interface conditions; proteins;

D O I：

10.1007/BF02491738

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In recent years, the hyphenation of capillary electrophoresis (CE) and electrospray ionization mass spectrometry (ESI-MS) has been widely used for the analysis of biological molecules with high efficiency and high accuracy However, the signal intensity of CE-ESI-MS is restricted by various parameters. This paper reports the effects on the CE-ESI-MS signal of the pH and the electrolyte concentration, the formic acid concentration of the sheath liquid, and the sheath liquid composition, using several proteins as samples. The study was performed systematically by experimental and theoretical analyses. The maximum signal intensity of three proteins (cytochrome c, insulin, bovine serum albumin) was attained with a pH 4.40 buffer containing 75 mM formic acid and 75 mM ammonium acetate. Investigation of the influence of the formic acid concentration in the sheath liquid (over the range of 0%-1%) on the ESI-MS signal of brovine serum albumin showed that the feasible amount of the formic acid in sheath liquid could improve the signal intensity of the sample ions. However, considerable bond broadening was observed that should be attributed mainly to column overloading, band spreading at the interface, and scanning data acquisition.

引用

页码：617 / 621

页数：5

共 30 条

[1] MICRO-ELECTROSPRAY - ZEPTOMOLE-ATTOMOLE PER MICROLITER SENSITIVITY FOR PEPTIDES [J].