A new algorithm for high-resolution reconstruction of single particles by electron microscopy

被引：22

作者：

Sorzano, C. O. S. ^{[1
,2
]}

Vargas, J. ^{[3
]}

de la Rosa-Trevin, J. M. ^{[4
]}

Jimenez, A. ^{[1
]}

Maluenda, D. ^{[1
]}

Melero, R. ^{[1
]}

Martinez, M. ^{[1
]}

Ramirez-Aportela, E. ^{[1
]}

Conesa, P. ^{[1
]}

Vilas, J. L. ^{[1
]}

Marabini, R. ^{[5
]}

Carazo, J. M. ^{[1
]}

机构：

[1] CSIC, Ctr Nac Biotecnol, Madrid 28049, Spain

[2] Univ San Pablo, CEU, Campus Urb Monteprincipe, Madrid 28668, Spain

[3] McGill Univ, Dept Anat & Cell Biol, Montreal, PQ, Canada

[4] SciLife Lab, Stockholm, Sweden

[5] Univ Autonoma Madrid, E-28049 Madrid, Spain

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2018年 / 204卷 / 02期

关键词：

Image processing; 3D reconstruction; Angular assignment; Volume restoration; EM STRUCTURE DETERMINATION; 3D CRYOELECTRON MICROSCOPY; CONTRAST TRANSFER-FUNCTION; CRYO-EM; 3-DIMENSIONAL RECONSTRUCTION; REFINEMENT; MODEL; REGISTRATION; VALIDATION; IMAGES;

D O I：

10.1016/j.jsb.2018.08.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Map Challenge organized by the Electron Microscopy Data Bank has prompted the development of an Xmipp high resolution reconstruction protocol (which we will refer to as highres) that is integrated in the software platform scipion. In this work we describe the details of the image angular alignment and map reconstruction steps in our new method. This algorithm is similar to the standard projection matching approach with some important modifications, especially in the area of detecting significant features in the reconstructed volume. We show that the new method is able to produce higher resolution maps than the current de facto standard as measured by the Fourier Shell Correlation, the Monogenic Local Resolution and EMRinger.

引用

页码：329 / 337

页数：9

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