Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons

被引：23

作者：

Miazzi, Fabio ^{[1
]}

Hansson, Bill S. ^{[1
]}

Wicher, Dieter ^{[1
]}

机构：

[1] Max Planck Inst Chem Ecol, Dept Evolutionary Neuroethol, Hans Knoll Str 8, D-07745 Jena, Germany

来源：

JOURNAL OF EXPERIMENTAL BIOLOGY | 2016年 / 219卷 / 12期

关键词：

3; 5 '-Cyclic adenosine monophosphate; Fruit fly; Odorant receptor; Orco; Or22a; Epac1-camps; IN-VIVO; TRANSDUCTION; RECEPTORS; CHANNELS; NETWORK;

D O I：

10.1242/jeb.137901

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Insect odorant receptors are seven transmembrane domain proteins that form cation channels, whose functional properties such as receptor sensitivity are subject to regulation by intracellular signaling cascades. Here, we used the cAMP fluorescent indicator Epac1-camps to investigate the occurrence of odor-induced cAMP production in olfactory sensory neurons (OSNs) of Drosophila melanogaster. We show that stimulation of the receptor complex with an odor mixture or with the synthetic agonist VUAA1 induces a cAMP response. Moreover, we show that while the intracellular Ca2+ concentration influences cAMP production, the OSN-specific receptor OrX is necessary to elicit cAMP responses in Ca2+-free conditions. These results provide direct evidence of a relationship between odorant receptor stimulation and cAMP production in olfactory sensory neurons in the fruit fly antenna and show that this method can be used to further investigate the role that this second messenger plays in insect olfaction.

引用

页码：1798 / 1803

页数：6