Mesenchymal stem cells up-regulate the invasive potential of prostate cancer cells via the eotaxin-3/CCR3 axis

被引：10

作者：

Ishida, Yukako ^{[1
]}

Kido, Akira ^{[1
]}

Akahane, Manabu ^{[2
]}

Kishi, Shingo ^{[3
]}

Tsukamoto, Shinji ^{[1
,3
]}

Fujii, Hiromasa ^{[3
]}

Honoki, Kanya ^{[3
]}

Tanaka, Yasuhito ^{[3
]}

机构：

[1] Nara Med Univ, Dept Rehabil Med, 840 Shijo Cho, Kashihara, Nara 6348522, Japan

[2] Nara Med Univ, Dept Publ Hlth Hlth Management & Policy, Nara, Japan

[3] Nara Med Univ, Dept Orthoped Surg, Nara, Japan

来源：

PATHOLOGY RESEARCH AND PRACTICE | 2018年 / 214卷 / 09期

关键词：

Mesenchymal stem cells; Cancer microenvironment; Eotaxin-3; CCR3; Homing chemokines; BONE-MARROW; CHEMOKINE RECEPTORS; ENGRAFTMENT; MIGRATION; EXPRESS; TRANSPLANTATION; EOSINOPHILS; PROGRESSION; ADHESION; GROWTH;

D O I：

10.1016/j.prp.2018.06.012

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

This study aimed to clarify the role of mesenchymal stem cells (MSCs) as a component of the cancer micro-environment. We investigated the homing-related chemokine expression levels of MSCs treated with a prostate cancer cell line (PC-3) -conditioned medium. Among several homing chemokines, an antibody array revealed that expression of eotaxin-3 (but not eotxin-1 and -2) was highly enhanced in MSCs treated with PC-3-conditioned medium. A gene expression array showed significantly increased expression of CCR3, a receptor of eotaxin-3, in PC-3. In a matrigel invasion assay, interferon-gamma, a specific inhibitor of eotaxin-related homing, significantly reduced the transmigration of PC-3 cells, under co-cultured condition with MSCs, in a dose-dependent manner (P < 0.05). Consistent with these results, anti-CCR3 antibody successfully reduced PC-3 migration under the co-cultured condition. These findings suggest that MSCs to modulation of the invasive potential of prostate cancer cells via the eotaxin-3/CCR3 axis.

引用

页码：1297 / 1302

页数：6

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