Mesenchymal stem cells up-regulate the invasive potential of prostate cancer cells via the eotaxin-3/CCR3 axis

被引：10

作者：

Ishida, Yukako ^{[1
]}

Kido, Akira ^{[1
]}

Akahane, Manabu ^{[2
]}

Kishi, Shingo ^{[3
]}

Tsukamoto, Shinji ^{[1
,3
]}

Fujii, Hiromasa ^{[3
]}

Honoki, Kanya ^{[3
]}

Tanaka, Yasuhito ^{[3
]}

机构：

[1] Nara Med Univ, Dept Rehabil Med, 840 Shijo Cho, Kashihara, Nara 6348522, Japan

[2] Nara Med Univ, Dept Publ Hlth Hlth Management & Policy, Nara, Japan

[3] Nara Med Univ, Dept Orthoped Surg, Nara, Japan

来源：

PATHOLOGY RESEARCH AND PRACTICE | 2018年 / 214卷 / 09期

关键词：

Mesenchymal stem cells; Cancer microenvironment; Eotaxin-3; CCR3; Homing chemokines; BONE-MARROW; CHEMOKINE RECEPTORS; ENGRAFTMENT; MIGRATION; EXPRESS; TRANSPLANTATION; EOSINOPHILS; PROGRESSION; ADHESION; GROWTH;

D O I：

10.1016/j.prp.2018.06.012

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

This study aimed to clarify the role of mesenchymal stem cells (MSCs) as a component of the cancer micro-environment. We investigated the homing-related chemokine expression levels of MSCs treated with a prostate cancer cell line (PC-3) -conditioned medium. Among several homing chemokines, an antibody array revealed that expression of eotaxin-3 (but not eotxin-1 and -2) was highly enhanced in MSCs treated with PC-3-conditioned medium. A gene expression array showed significantly increased expression of CCR3, a receptor of eotaxin-3, in PC-3. In a matrigel invasion assay, interferon-gamma, a specific inhibitor of eotaxin-related homing, significantly reduced the transmigration of PC-3 cells, under co-cultured condition with MSCs, in a dose-dependent manner (P < 0.05). Consistent with these results, anti-CCR3 antibody successfully reduced PC-3 migration under the co-cultured condition. These findings suggest that MSCs to modulation of the invasive potential of prostate cancer cells via the eotaxin-3/CCR3 axis.

引用

页码：1297 / 1302

页数：6

共 28 条

[1] CCL11 (eotaxin-1): A new diagnostic serum marker for prostate cancer [J].

Agarwal, Manisha ;

He, Chang ;

Siddiqui, Javed ;

Wei, John T. ;

Macoska, Jill A. .

PROSTATE, 2013, 73 (06) :573-581

[2] Chemokine/chemokine receptor nomenclature [J].

Bacon, K ;

Baggiolini, M ;

Broxmeyer, H ;

Horuk, R ;

Lindley, I ;

Mantovani, A ;

Matsushima, K ;

Murphy, P ;

Nomiyama, H ;

Oppenheim, J ;

Rot, A ;

Schall, T ;

Tsang, M ;

Thorpe, R ;

Van Damme, J ;

Wadhwa, M ;

Yoshie, O ;

Zlotnik, A ;

Zoon, K .

CYTOKINE, 2003, 21 (01) :48-49

[3] Bone marrow-derived mesenchymal stem cells and the tumor microenvironment [J].

Bergfeld, Scott A. ;

DeClerck, Yves A. .

CANCER AND METASTASIS REVIEWS, 2010, 29 (02) :249-261

[4]

Campbell EM, 1998, J IMMUNOL, V161, P7047

[5] Autologous mesenchymal stem cell treatment increased T regulatory cells with no effect on disease activity in two systemic lupus erythematosus patients [J].

Carrion, F. ;

Nova, E. ;

Ruiz, C. ;

Diaz, F. ;

Inostroza, C. ;

Rojo, D. ;

Monckeberg, G. ;

Figueroa, F. E. .

LUPUS, 2010, 19 (03) :317-322

[6] Human mesenchymal stem cells constitutively express chemokines and chemokine receptors that can be upregulated by cytokines, IFN-β, and Copaxone [J].

Croitoru-Lamoury, Juliana ;

Lamoury, Francois M. J. ;

Zaunders, John J. ;

Veas, Laura A. ;

Brew, Bruce J. .

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 2007, 27 (01) :53-64

[7] Th2-mediated anti-tumour immunity: friend or foe? [J].

Ellyard, J. I. ;

Simson, L. ;

Parish, C. R. .

TISSUE ANTIGENS, 2007, 70 (01) :1-11

[8] Engraftment of allogeneic mesenchymal stem cells in the bone marrow of a patient with severe idiopathic aplastic anemia improves stroma [J].

Fouillard, L ;

Bensidhoum, M ;

Bories, D ;

Bonte, H ;

Lopez, M ;

Moseley, AM ;

Smith, A ;

Lesage, S ;

Beaujean, F ;

Thierry, D ;

Gourmelon, P ;

Najman, A ;

Gorin, NC .

LEUKEMIA, 2003, 17 (02) :474-476

[9] Inhibition of prostate cancer cell growth by activated eosinophils [J].

Furbert-Harris, P ;

Parish-Gause, D ;

Laniyan, I ;

Hunter, KA ;

Okomo-Awich, J ;

Vaughn, TR ;

Forrest, KC ;

Howland, C ;

Abdelnaby, A ;

Oredipe, OA .

PROSTATE, 2003, 57 (02) :165-175

[10] Involvement of eosinophils in the anti-tumor response [J].

Gatault, Solene ;

Legrand, Fanny ;

Delbeke, Marie ;

Loiseau, Sylvie ;

Capron, Monique .

CANCER IMMUNOLOGY IMMUNOTHERAPY, 2012, 61 (09) :1527-1534

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