Using photoactivatable fluorescent protein Dendra2 to track protein movement

被引：88
作者：
Chudakov, Dmitriy M. [1 ]
Lukyanov, Sergey [1 ]
Lukyanov, Konstantin A. [1 ]
机构：
[1] Russian Acad Sci, Inst Bioorgan Chem, Moscow, Russia
来源：
BIOTECHNIQUES | 2007年 / 42卷 / 05期
关键词：
D O I：
10.2144/000112470
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly fluorescent state. Several types of such proteins were developed recently, and some of them are already becoming popular tools to study protein mobility. Here we provide detailed recommendations on application of the monomeric green-to-red photoconvertible fluorescent protein Dendra2 for protein tracking in living cultured cells.
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页码：553 / +
页数：7
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