Engineering Resistance Against Mungbean yellow mosaic India virus Using Antisense RNA

被引：25
作者：
Haq, Q. M. I. [1 ]
Ali, Arif [2 ]
Malathi, V. G. [1 ]
机构：
[1] Indian Agr Res Inst, Adv Ctr Plant Virol, Div Plant Pathol, New Delhi 110012, India
[2] Jamia Millia Islamia, Dept Biotechnol, New Delhi 110025, India
来源：
INDIAN JOURNAL OF VIROLOGY | 2010年 / 21卷 / 01期
关键词：
MYMIV isolate; Agroinoculation; Legumes; TRANSGENIC TOBACCO; DNA VIRUS; GEMINIVIRUS; GENE; REPLICATION; EXPRESSION; PLANTS;
D O I：
10.1007/s13337-010-0003-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Yellow mosaic disease of cultivated legumes in South-East Asia, is caused by Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV) belonging to the genus Begomovirus of the family Geminiviridae. Efforts to engineer resistance against the genus Begomovirus are focused mainly on silencing of complementary-sense virus genes involved in virus replication. Here we have targeted a complementary-sense gene (ACI) encoding Replication initiation Protein (Rep) to develop resistance against soybean isolate of Mungbean yellow mosaic India virus-[India: New Delhi: Soybean 2: 1999], a bipartite begomovirus prevalent throughout the Indian subcontinent. We show that the legume host plants co-agroinoculated with infectious constructs of soybean isolate of Mungbean yellow mosaic India virus [India: New Delhi: Soybean 2: 1999] along with this antisense Rep gene construct show resistance to the virus.
引用
收藏
页码：82 / 85
页数：4
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