Curcumin inhibits the expression of proinflammatory mediators and MMP-9 in gingival epithelial cells stimulated for a prolonged period with lipopolysaccharides derived from Porphyromonas gingivalis

被引：12

作者：

Toraya, Seiko ^{[1
]}

Uehara, Osamu ^{[1
]}

Hiraki, Daichi ^{[2
]}

Harada, Fumiya ^{[3
]}

Neopane, Puja ^{[2
]}

Morikawa, Tetsuro ^{[2
]}

Takai, Rie ^{[4
]}

Yoshida, Koki ^{[2
]}

Matsuoka, Hirofumi ^{[2
]}

Kitaichi, Nobuyoshi ^{[5
]}

Chiba, Itsuo ^{[1
]}

Abiko, Yoshihiro ^{[2
]}

机构：

[1] Hlth Sci Univ Hokkaido, Sch Dent, Dept Oral Growth & Dev, Div Dis Control & Mol Epidemiol, 1757 Kanazawa, Ishikari, Hokkaido 0610293, Japan

[2] Hlth Sci Univ Hokkaido, Sch Dent, Dept Human Biol & Pathophysiol, Div Oral Med & Pathol, 1757 Kanazawa, Ishikari, Hokkaido 0610293, Japan

[3] Hlth Sci Univ Hokkaido, Sch Dent, Dept Human Biol & Pathophysiol, Div Oral & Maxillofacial Surg, 1757 Kanazawa, Ishikari, Hokkaido 0610293, Japan

[4] Hlth Sci Univ Hokkaido, Res Inst Hlth Sci, 1757 Kanazawa, Ishikari, Hokkaido 0610293, Japan

[5] Hokkaido Hosp, Hlth Sci Univ, Dept Ophthalmol, Kita Ku, 2-5 Ainosato, Sapporo, Hokkaido 0028072, Japan

来源：

ODONTOLOGY | 2020年 / 108卷 / 01期

关键词：

P; gingivalis; Lipopolysaccharide; Curcumin; MMP-9; Gingival epithelial cells; KAPPA-B; PERIODONTITIS; ACTIVATION; FIBROBLASTS; MODULATION; MIGRATION; PATHWAY;

D O I：

10.1007/s10266-019-00432-8

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Curcumin, a yellow phytochemical found in the rhizomes of Curcuma longa, has various biological effects, including anti-oxidant and anti-inflammatory activities. In the present study, we examined the effect of curcumin on the expression of inflammatory cytokines in human gingival epithelial progenitor cells (HGEPs) stimulated for a prolonged period with lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. The cells were alternately cultured with LPS and/or curcumin every 3 days for 18 days. The expression levels of TNF-alpha, IL-1 beta, IL-6, TIMP-1, and MMP-9 in the HGEPs were evaluated by quantitative real-time polymerase chain reaction. Enzyme-linked immunosorbent assay was used to measure the concentrations of these five proteins in the supernatant and nuclear factor (NF)-kappa B in the nuclear extracts. Curcumin inhibited the mRNA expression levels of TNF-alpha, IL-1 beta, IL-6, and MMP-9 in HGEPs treated with curcumin over a prolonged period. Similarly, the expression levels of IL-1 beta, IL-6, and MMP-9 were decreased in the culture supernatants. NF-kappa B activity was also inhibited in the cells cultured with curcumin. In conclusion, these findings indicate that curcumin inhibits the expression of inflammatory cytokines and MMP-9 in primary gingival epithelial cells stimulated with P. gingivalis-derived LPS via NF-kappa B activation.

引用

页码：16 / 24

页数：9

共 32 条

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