The use of FTIR spectroscopy to assess quantitative changes in the biochemical composition of microalgae

被引：110

作者：

Wagner, Heiko ^{[1
]}

Liu, Zhixin ^{[1
]}

Langner, Uwe ^{[2
]}

Stehfest, Katja ^{[3
]}

Wilhelm, Christian ^{[1
]}

机构：

[1] Univ Leipzig, Inst Biol 1, Dept Plant Physiol, D-04103 Leipzig, Germany

[2] Univ Halle Wittenberg, Inst Biol Plant Physiol, D-06120 Halle, Saale, Germany

[3] Humboldt Univ, Inst Biol, Dept Expt Biophys, D-10115 Berlin, Germany

来源：

JOURNAL OF BIOPHOTONICS | 2010年 / 3卷 / 8-9期

关键词：

FTIR spectroscopy; macromolecules; algal physiology; phytoplankton; Chlamydomonas reinhardtii; TRANSFORM INFRARED-SPECTROSCOPY; NUTRIENT-LIMITED GROWTH; PHYTOPLANKTON GROWTH; BIOMASS COMPOSITION; ENERGY-BALANCE; NITRATE; RATIOS; LIGHT; TOOL; PHAEODACTYLUM;

D O I：

10.1002/jbio.201000019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A mid-infrared spectroscopic method was developed for the simultaneous and quantitative determination of total protein, carbohydrate and lipid contents of microalgal cells. Based on a chemometric approach, measured FTIR (Fourier transform infrared) spectra from algal cells were reconstructed by a partial least square algorithm, using the spectra of the reference substances to determine their relative contribution to the overall cell spectrum. From this specific absorption, absolute macromolecular cell composition [pg cell(-1)] can be calculated using calibration curves, which have been validated by independent biochemical methods. The future potential of this method for photosynthesis research is shown by its application to follow time-resolved changes in the cellular composition of microalgae during an illumination period of several hours. We show how the macromolecular composition can be investigated by FTIR spectroscopy methods. This can substantially increase the efficiency of screening processes like bioreactor monitoring and may be beneficial in metabolic engineering of algal cells. [GRAPHICS] FTIR spectra (a) of whole C. reinhardtii cells and (b) of reference substances.

引用

页码：557 / 566

页数：10

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