Long-term response toward inorganic carbon limitation in wild type and glycolate turnover mutants of the cyanobacterium Synechocystis sp strain PCC 68031[W]

被引:114
作者
Eisenhut, Marion
Aguirre von Wobeser, Eneas
Jonas, Ludwig
Schubert, Hendrik
Ibelings, Bas W.
Bauwe, Hermann
Matthijs, Hans C. P.
Hagemann, Martin [1 ]
机构
[1] Univ Rostock, Inst Biowissensch, Abt Pflanzenphysiol & Okol, D-18059 Rostock, Germany
[2] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, NL-1018 WS Amsterdam, Netherlands
[3] Univ Rostock, Inst Pathol, Elektronenmikroskop Zentrum, D-18055 Rostock, Germany
[4] Netherlands Inst Ecol, Ctr Limnol, Dept Foodweb Studies, NL-3631 AC Nieuwersluis, Netherlands
[5] Swiss Fed Inst Aquat Sci & Technol, Ctr Ecol Evolut & Biogeochem, CH-6047 Kastanienbaum, Switzerland
关键词
D O I
10.1104/pp.107.103341
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Concerted changes in the transcriptional pattern and physiological traits that result from long-term (here defined as up to 24 h) limitation of inorganic carbon (C-i) have been investigated for the cyanobacterium Synechocystis sp. strain PCC 6803. Results from reverse transcription-polymerase chain reaction and genome-wide DNA microarray analyses indicated stable up-regulation of genes for inducible CO2 and HCO3- uptake systems and of the rfb cluster that encodes enzymes involved in outer cell wall polysaccharide synthesis. Coordinated up-regulation of photosystem I genes was further found and supported by a higher photosystem I content and activity under low C-i (LC) conditions. Bacterial-type glycerate pathway genes were induced by LC conditions, in contrast to the genes for the plant-like photorespiratory C-2 cycle. Down-regulation was observed for nitrate assimilation genes and surprisingly also for almost all carboxysomal proteins. However, for the latter the observed elongation of the half-life time of the large subunit of Rubisco protein may render compensation. Mutants defective in glycolate turnover (Delta glcD and Delta gcvT) showed some transcriptional changes under high C-i conditions that are characteristic for LC conditions in wild-type cells, like a modest down-regulation of carboxysomal genes. Properties under LC conditions were comparable to LC wild type, including the strong response of genes encoding inducible high-affinity C-i uptake systems. Electron microscopy revealed a conspicuous increase in number of carboxysomes per cell in mutant Delta glcD already under high C-i conditions. These data indicate that an increased level of photorespiratory intermediates may affect carboxysomal components but does not intervene with the expression of majority of LC inducible genes.
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页码:1946 / 1959
页数:14
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