Amylase measurement with 2-chloro-4-nitrophenyl maltotrioside as substrate

被引：50

作者：

Foo, AY

Bais, R

机构：

[1] Royal Free Hosp, Dept Chem Pathol, London NW3 2QG, England

[2] Royal N Shore Hosp, Dept Chem Pathol, Pacific Lab Med Serv, St Leonards, NSW 2065, Australia

来源：

CLINICA CHIMICA ACTA | 1998年 / 272卷 / 02期

关键词：

measurement of amylase activity; method comparison; 2-chloro-4-nitrophenyl maltotrioside (CNP-G3);

D O I：

10.1016/S0009-8981(98)00009-6

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The use of 2-chloro-4-nitrophenyl maltotrioside (CNP-GS) as substrate to measure amylase (EC 3.2.1.1) activity in serum directly without the use of auxiliary enzymes was evaluated at two centres. The method was precise (within-run C.V. < 2% and between-run CN. < 3%), there was no lag phase, background absorbance was low and there were minimal effects of pH changes. When compared with a method which uses 4,6-ethylidene (G(7))-p-nitrophenyl (G(1))-alpha-D-maltoheptaoside (EPS-G7) as substrate, the CNP-G3 method had greater sensitivity and longer reagent stability (21 days compared with 2 days at 4 degrees C). The activity measured with the CNP-G3 method correlated well with methods using either EPS-G7 and maltotetraose as substrates. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：137 / 147

页数：11

共 13 条

[1]

BADENOCH JL, 1989, CLIN CHEM, V35, P645

[2]

ECKFELDT JH, 1989, CLIN LAB MED, V9, P732

[3] AMYLASE MEASUREMENT - WHICH METHOD [J].