Developmental exposures to ethanol or dimethylsulfoxide at low concentrations alter locomotor activity in larval zebrafish: Implications for behavioral toxicity bioassays

被引:100
作者
Chen, Te-Hao [1 ,2 ]
Wang, Yen-Hsin [2 ]
Wu, Yu-Hwan [3 ]
机构
[1] Natl Museum Marine Biol & Aquarium, Dept Biol, Checheng, Pingtung, Taiwan
[2] Natl Dong Hwa Univ, Inst Marine Biodivers & Evolutionary Biol, Checheng, Pingtung, Taiwan
[3] Univ Calif Davis, Coll Biol Sci, Davis, CA 95616 USA
关键词
Solvents; Ethanol; Dimethylsulfoxide; Zebrafish; Behavior; Bioassay; DANIO-RERIO; CHLORPYRIFOS EXPOSURE; MODEL; SYSTEM; FISH; ALCOHOL; DMSO;
D O I
10.1016/j.aquatox.2011.01.010
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
Ethanol and dimethylsulfoxide (DMSO) are commonly used as carrier solvents for lipophilic chemicals in aquatic toxicity bioassays. However, very little information has been reported on the behavioral effects of these solvents. In this study, we examined the effects of ethanol and DMSO on development and locomotor activity by a zebrafish embryo-larval bioassay. The zebrafish were exposed to different concentrations (control, 0.01, 0.1, and 1%) of ethanol or DMSO from blastula stage to 144 hour-post-fertilization (hpf). Hatchability, survival, and abnormalities were monitored every 12 h, and locomotor activity of the larvae was analyzed at 144 hpf. Hatchability was not affected by the ethanol or DMSO treatments. No effect on survival was observed except the 1% ethanol group suffered 89% mortality during 108-120 hpf. No developmental defects were observed in any of the solvents at the 0.01 and 0.1% concentrations, but significantly higher deformity rates occurred with 1% ethanol and DMSO groups. Hyperactivity and less tortuous swimming paths were observed in all ethanol and DMSO concentrations. Based on this study, we suggest that data of behavioral toxicity bioassays using ethanol or DMSO as carrier solvents should be interpreted cautiously, because the solvents at low concentrations could alter locomotor activity of larval zebrafish without causing any observable developmental defects. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:162 / 166
页数:5
相关论文
共 22 条
[1]   Behavioural assessment of dimethylsulfoxide neurotoxicity in rats [J].
Authier, N ;
Dupuis, E ;
Kwasiborski, A ;
Eschalier, A ;
Coudoré, F .
TOXICOLOGY LETTERS, 2002, 132 (02) :117-121
[2]   An endocrine disrupter increases growth and risky behavior in threespined stickleback (Gasterosteus aculeatus) [J].
Bell, AM .
HORMONES AND BEHAVIOR, 2004, 45 (02) :108-114
[3]   BEHAVIORAL-EFFECTS OF VEHICLES - DMSO, ETHANOL, TWEEN-20, TWEEN-80, AND EMULPHOR-620 [J].
CASTRO, CA ;
HOGAN, JB ;
BENSON, KA ;
SHEHATA, CW ;
LANDAUER, MR .
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 1995, 50 (04) :521-526
[4]   Effect on the peripheral nervous system of systemically administered dimethylsulfoxide in the rat: a neurophysiological and pathological study [J].
Cavaletti, G ;
Oggioni, N ;
Sala, F ;
Pezzoni, G ;
Cavalletti, E ;
Marmiroli, P ;
Petruccioli, MG ;
Frattola, L ;
Tredici, G .
TOXICOLOGY LETTERS, 2000, 118 (1-2) :103-107
[5]   Chronic exposure of 2,2′,4,4′-tetrabromodiphenyl ether (PBDE-47) alters locomotion behavior in juvenile zebrafish (Danio rerio) [J].
Chou, Chun-Ting ;
Hsiao, Yu-Chen ;
Ko, Fung-Chi ;
Cheng, Jing-O ;
Cheng, Ying-Ming ;
Chen, Te-Hao .
AQUATIC TOXICOLOGY, 2010, 98 (04) :388-395
[6]   Behavioral changes in fish exposed to phytoestrogens [J].
Clotfelter, Ethan D. ;
Rodriguez, Alison C. .
ENVIRONMENTAL POLLUTION, 2006, 144 (03) :833-839
[7]   Automated analysis of behavior in zebrafish larvae [J].
Creton, Robbert .
BEHAVIOURAL BRAIN RESEARCH, 2009, 203 (01) :127-136
[8]  
Eaton J., 1975, METHODS ACUTE TOXICI
[9]   Zebrafish provide a sensitive model of persisting neurobehavioral effects of developmental chlorpyrifos exposure: Comparison with nicotine and pilocarpine effects and relationship to dopamine deficits [J].
Eddins, Donnie ;
Cerutti, Daniel ;
Williams, Paul ;
Linney, Elwood ;
Levin, Edward D. .
NEUROTOXICOLOGY AND TERATOLOGY, 2010, 32 (01) :99-108
[10]   DIMETHYLSULFOXIDE (DMSO) BLOCKS CONDUCTION IN PERIPHERAL-NERVE C-FIBERS - A POSSIBLE MECHANISM OF ANALGESIA [J].
EVANS, MS ;
REID, KH ;
SHARP, JB .
NEUROSCIENCE LETTERS, 1993, 150 (02) :145-148