A diffusion NMR method for the prediction of the weight-average molecular weight of globular proteins in aqueous media of different viscosities

被引：5

作者：

Arrabal-Campos, Francisco M. ^{[1
]}

Aguilera-Saez, Luis M. ^{[1
]}

Fernandez, Ignacio ^{[1
]}

机构：

[1] Univ Almeria, Res Ctr CIAIMBITAL, Dept Chem & Phys, Ctra Sacramento S-N, Almeria 04120, Spain

来源：

ANALYTICAL METHODS | 2019年 / 11卷 / 02期

关键词：

SELF-DIFFUSION; DEPENDENCE; COEFFICIENTS; SIZE;

D O I：

10.1039/c8ay01817k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have introduced for the first time, a non-viscosity dependent universal calibration curve (UCC) for the successful prediction of the weight-average molecular weights of globular proteins in the range of 8.5-66.2 kDa with no dependence on the solvent viscosity. Plots of log(Dh) versus log M-w showed excellent linearity (R-2 > 0.99) within four different methods (LMS, TRAIn, NNLS and ITAMeD). To demonstrate the applicability of this new UCC curve, we have proposed as a proof of concept the determination of the M-w of myoglobin from an equine heart in deuterated water of four different viscosity values. The corresponding (D-eta) values were fitted to the UCC to calculate their corresponding weight-average M-w that was in all the cases centered at 17 321.2 Da, with differences in the mass below 5.7% with respect to the commercial value.

引用

页码：142 / 147

页数：6

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