Integrated microfabricated systems including a purification module and an on-chip nano electrospray ionization interface for biological analysis

被引:38
作者
Carlier, J
Arscott, S
Thomy, V
Camart, JC
Cren-Olivé, C
Le Gac, S
机构
[1] Univ Sci & Technol, Lab Chim Organ & Macromol, CNRS, UMR 8009, F-59655 Villeneuve Dascq, France
[2] Univ Sci & Technol, CNRS, UMR 8520, Inst Elect Microelect & Nanotechnol, Villeneuve Dascq, France
关键词
microfluidics; integrated microsystems; sample preparation; mass spectrometry ESI-MS; monolithic phase; protein analysis;
D O I
10.1016/j.chroma.2004.12.037
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We report here on an integrated microfabricated device dedicated to the preparation of biological samples prior to their on-line analysis by electrospray ionization-mass spectrometry (ESI-MS). This microfluidic device is fabricated using the negative photoresist SU-8 by microtechnology techniques. The device includes a chromatographic module plus an ESI interface for MS. The chromatographic module is dedicated to sample purification and is based on a polymer monolithic phase which includes hydrophobic moieties. The ESI interface is integrated onto the chip and is based on a capillary slot. We present here the integration of these different modules onto a single system that is fabricated via a SU-8-based microtechnology route. We present also their testing for the purification of peptide samples. This started with a partial integration step with the combination of at least two of the modules (microsystem + monolith; microsystem + nib) and their test before the fabrication and testing of fully integrated microsystems. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:213 / 222
页数:10
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