Massilia solisilvae sp nov., Massilia terrae sp nov and Massilia agilis sp nov., isolated from forest soil in South Korea by using a newly developed culture method

Using a newly developed culture method for not yet cultured soil bacteria, three Gram-stain-negative, aerobic, non-sporeforming, motile, and rod-shaped bacteria (strain designated J18(T), J11(T) and J9(T)) were isolated from forest soil at Kyonggi University, South Korea. Isolates were subjected to a taxonomic study by using a polyphasic approach. According to a phylogenetic tree based on 16S rRNA gene sequences, strains J18(T), J11(T) and J9(T) belonged to the genus Massilia and clustered with Massilia haematophila CCUG 38318(T) (similarity range: 97.6 +/- 98.0 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, and the genomic DNA G+ C contents of strains J18(T), J11(T) and J9(T) were 63.4, 68.7 and 64.5 mol%, respectively. The major polyamines were putrescine and 2hydroxyputescine, which were detected in all three strains. DNA-DNA between the three tested strains and the reference strains much lower than 70 %, the recommended threshold value for the delineation of genomic species. The predominant respiratory quinine was ubiquinone-8 (Q-8) and the major cellular fatty acids were Summed feature 3 (C-16 : 1 omega 6c/C-16 : 1 omega 7c) and C-16 : 0. On the basis of phenotypic and genotypic data and DNA-DNA hybridization results, the three isolates are considered to represent three novel species of the genus Massilia, for which the names Massilia solisilvae sp. nov. for type strain J18(T) (= KEMB 9005-366(T) = JCM 31607(T)), Massilia terrae sp. nov. for type strain J11(T) (= KEMB 9005-360(T) = JCM 31606 T) and Massilia agilis sp. nov. for type strain J9(T) (= KEMB 9005-359(T) = JCM 31605(T)) are proposed.