Molecular cytogenetic characterization of stem-like cancer cells isolated from established cell lines

被引：7

作者：

Gasparini, Patrizia ^{[1
]}

Bertolini, Giulia ^{[1
]}

Binda, Mara ^{[2
]}

Magnifico, Alessandra ^{[3
]}

Albano, Luisa ^{[3
]}

Tortoreto, Monica ^{[4
]}

Pratesi, Graziella ^{[4
]}

Facchinetti, Federica ^{[1
]}

Abolafio, Gabriella ^{[5
]}

Roz, Luca ^{[1
]}

Tagliabue, Elda ^{[3
]}

Daidone, Maria Grazia ^{[2
]}

Sozzi, Gabriella ^{[1
]}

机构：

[1] Fdn IRCCS, Dept Expt Oncol & Labs, Mol Cytogenet Unit, Ist Nazl Tumori, I-20133 Milan, Italy

[2] Fdn IRCCS, Translat Res Unit, Ist Nazl Tumori, I-20133 Milan, Italy

[3] Fdn IRCCS, Mol Targeting Unit, Ist Nazl Tumori, I-20133 Milan, Italy

[4] Fdn IRCCS, Preclin Chemotherapy & Pharmacol Unit, Ist Nazl Tumori, I-20133 Milan, Italy

[5] Fdn IRCCS, Dept Expt Oncol, Ist Nazl Tumori, I-20133 Milan, Italy

来源：

CANCER LETTERS | 2010年 / 296卷 / 02期

关键词：

Cancer stem cells; Cell lines; Chromosomal rearrangements; FISH; SKY; BREAST-CANCER; PROSPECTIVE IDENTIFICATION; INITIATING CELLS; BRAIN-TUMORS; GLIOBLASTOMAS; SUBPOPULATION; POPULATION;

D O I：

10.1016/j.canlet.2010.04.009

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

There is indication that tumor growth is sustained by subpopulation of cells with stem-like features but little is known on their genomic characterization and their genetic stability. We report a detailed molecular cytogenetic characterization using Spectral Karyotyping and fluorescent in situ hybridization of parental serum-cultured adherent cells and their sphere-growing stem-like counterpart before and after differentiation from six cell lines established from solid tumors. Our findings indicate increased cytogenetic complexity in sphere-growing stem-like and their differentiated adherent cells compared to parental adherent component suggesting the existence within cell lines of heterogeneous and genetically unstable subpopulations of cells endowed with stem-like features. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：206 / 215

页数：10

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