Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2β

被引：55

作者：

Cai, Xinjiang ^{[1
,3
]}

Srivastava, Shekhar ^{[1
,3
]}

Sun, Yi ^{[1
,3
]}

Li, Zhai ^{[1
,3
]}

Wu, Haiyan ^{[4
]}

Zuvela-Jelaska, Ljiljana ^{[5
]}

Li, Jun ^{[5
]}

Salamon, Rachel S. ^{[4
]}

Backer, Jonathan M. ^{[4
]}

Skolnik, Edward Y. ^{[1
,2
,3
]}

机构：

[1] NYU, Langone Med Ctr, Helen L & Martin S Kimmel Ctr Biol & Med, Skirball Inst Biomol Med, New York, NY 10016 USA

[2] NYU, Langone Med Ctr, Div Nephrol, Dept Med, New York, NY 10016 USA

[3] NYU, Langone Med Ctr, Dept Pharmacol, New York, NY 10016 USA

[4] Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461 USA

[5] Boehringer Ingelheim Pharmaceut Inc, Ridgefield, CT 06877 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 50期

基金：

美国国家卫生研究院;

关键词：

Ca2+ signaling; phosphatidylinositol-3; phosphate; T helper cells; tripartite motif containing protein 27 knockout; K+ CHANNEL KCA3.1; DIPHOSPHATE KINASE-B; PHOSPHOINOSITIDE; 3-KINASE; POTASSIUM CHANNEL; PHOSPHATIDYLINOSITOL; THERAPEUTIC TARGETS; ACTIVATION; DEGRADATION; FAMILY; IKCA1;

D O I：

10.1073/pnas.1111233109

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The K+ channel KCa3.1 is required for Ca2+ influx and the subsequent activation of CD4 T cells. The class II phosphatidylinositol 3 kinase C2 beta (PI3KC2 beta) is activated by the T-cell receptor (TCR) and is critical for KCa3.1 channel activation. Tripartite motif containing protein 27 (TRIM27) is a member of a large family of proteins that function as Really Interesting New Gene (RING) E3 ubiquitin ligases. We now show that TRIM27 functions as an E3 ligase and mediates lysine 48 polyubiquitination of PI3KC2 beta, leading to a decrease in PI3K enzyme activity. By inhibiting PI3KC2 beta, TRIM27 also functions to negatively regulate CD4 T cells by inhibiting KCa3.1 channel activity and TCR-stimulated Ca2+ influx and cytokine production in Jurkat, primary human CD4 T cells, and Th0, Th1, and Th2 CD4 T cells generated from TRIM27(-/-) mice. These findings provide a unique mechanism for regulating class II PI3Ks, and identify TRIM27 as a previously undescribed negative regulator of CD4 T cells.

引用

页码：20072 / 20077

页数：6

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