Degradation of methyl tert-butyl ether by gel immobilized Methylibium petroleiphilum PM1

被引：42

作者：

Chen, Dongzhi ^{[1
]}

Chen, Jianmeng ^{[1
]}

Zhong, Weihong ^{[1
]}

Cheng, Zhuowei ^{[1
]}

机构：

[1] Zhejiang Univ Technol, Coll Biolog & Environm Engn, Hangzhou 310032, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2008年 / 99卷 / 11期

基金：

中国国家自然科学基金;

关键词：

methyl tert-butyl ether; immobilization; alginate; groundwater; Methylibium petroleiphilum PM1;

D O I：

10.1016/j.biortech.2007.09.075

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Cells of Methylibium petroleiphilum PM1 were immobilized in gel beads to degrade methyl tert-butyl ether (MTBE). Calcium alginate, agar, polyacrylamide and polyvinvyl alcohol were screened as suitable immobilization matrices, with calcium alginate demonstrating the fastest MTBE-degradation rate. The rate was accelerated by 1.8-fold when the beads had been treated in physiological saline for 24 h at 28 degrees C. MTBE degradation in mineral salts medium (MSM) was accompanied by the increase of biomass. The half-life of MTBE-degradation activity for the encapsulated cells stored at 28 degrees C was about 120 h, which was obviously longer than that of free cells (approximately 36 h). Efficient reusability of the beads up to 30 batches was achieved in poor nutrition solution as compared to only 6 batches in MSM. The immobilized cells could be operated in a packed-bed reactor for degradation of 10 mg L(-1) MTBE in groundwater with more than 99% removal efficiency at hydraulic retention time of 20 min. These results suggested that immobilized cells of PM1 in bioreactor might be applicable to a groundwater treatment system for the removal of MTBE. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：4702 / 4708

页数：7

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