RAPD fingerprinting for genotypic differentiation of S. aureus clinical isolates from camel (Camelus dromedarius)

Randomly amplified polymorphic DNA (RAPD) typing was performed on 21 S. aureus isolates from clinical wounds and abscesses in camel. Out of ten primers chosen for screening to know discrimination ability for S. aureus isolates three primers namely opc 1,2 and 10 showed good discriminatory index and generated 151 polymorphic bands with size ranging from 200-2500 base pairs. All the isolates were classified into two groups. Group 1 contained only one isolate while rest 20 were in group 2. The study indicated that there is much more genetic variation than studied earlier and RAPD can successfully be applied for differentiation of S. aureus strains.