Growth of Lactobacillus plantarum in media containing hydrolysates of fish viscera

被引:58
作者
Horn, SJ
Aspmo, SI
Eijsink, VGH
机构
[1] Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, N-1432 As, Norway
[2] Maritex AS, Sortland, Norway
关键词
cod; fish hydrolysate; growth medium; lactic acid; Lactobacillus; NC8;
D O I
10.1111/j.1365-2672.2005.02702.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To compare growth of Lactobacillus plantarum on media containing hydrolysates ( peptones) from cod viscera with growth on commercial media. Methods and Results: Growth of Lact. plantarum on various fish peptones and commercial peptones/ extracts was evaluated using both a Bioscreen apparatus ( microtiter plates, no pH control) and fermentors ( with pH control). Generally, the performance of the fish peptones was good and only beaten by the performance of yeast extract. Replacement of the 22 g l(-1) complex nitrogen source in standard MRS medium with only 5 g l(-1) fish peptone reduced the biomass yield with only 10%, whereas replacement with a mixture of 2.5 g l(-1) fish peptone and 2.5 g l(-1) yeast extract increased the biomass yield by 10%. Conclusions: Peptones derived from cod viscera support excellent growth of Lact. plantarum. Significance and Impact of the Study: We show that peptones derived from cod viscera are promising constituents of growth media for fastidious food bacteria such as lactobacilli. Media containing these peptones show excellent performance while problems associated with the use of meat- derived peptones ( BSE, kosher status) or plant- derived peptones ( genetically modified organisms) are avoided.
引用
收藏
页码:1082 / 1089
页数:8
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