Using the class 1 integron-integrase gene as a proxy for anthropogenic pollution

被引:1015
作者
Gillings, Michael R. [1 ]
Gaze, William H. [2 ]
Pruden, Amy [3 ]
Smalla, Kornelia [4 ]
Tiedje, James M. [5 ]
Zhu, Yong-Guan [6 ]
机构
[1] Macquarie Univ, Genes Geosci Res Ctr, Dept Biol Sci, Sydney, NSW 2109, Australia
[2] Univ Exeter, Royal Cornwall Hosp, Sch Med, European Ctr Environm & Human Hlth, Truro, England
[3] Virginia Tech, Via Dept Civil & Environm Engn, Blacksburg, VA USA
[4] Julius Kuhn Inst, Inst Epidemiol & Pathogen Diagnost, Fed Res Ctr Cultivated Plants, Braunschweig, Germany
[5] Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA
[6] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen, Peoples R China
基金
美国国家科学基金会; 澳大利亚研究理事会;
关键词
ANTIBIOTIC-RESISTANCE GENES; MUNICIPAL WASTE-WATER; ANTIMICROBIAL RESISTANCE; ESCHERICHIA-COLI; ENVIRONMENTAL-SAMPLES; BACTERIAL COMMUNITIES; COMMENSAL BACTERIA; METAL RESISTANCE; TREATMENT-PLANT; EASTERN CHINA;
D O I
10.1038/ismej.2014.226
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Around all human activity, there are zones of pollution with pesticides, heavy metals, pharmaceuticals, personal care products and the microorganisms associated with human waste streams and agriculture. This diversity of pollutants, whose concentration varies spatially and temporally, is a major challenge for monitoring. Here, we suggest that the relative abundance of the clinical class 1 integron-integrase gene, intI1, is a good proxy for pollution because: (1) intI1 is linked to genes conferring resistance to antibiotics, disinfectants and heavy metals; (2) it is found in a wide variety of pathogenic and nonpathogenic bacteria; (3) its abundance can change rapidly because its host cells can have rapid generation times and it can move between bacteria by horizontal gene transfer; and (4) a single DNA sequence variant of intI1 is now found on a wide diversity of xenogenetic elements, these being complex mosaic DNA elements fixed through the agency of human selection. Here we review the literature examining the relationship between anthropogenic impacts and the abundance of intI1, and outline an approach by which intI1 could serve as a proxy for anthropogenic pollution.
引用
收藏
页码:1269 / 1279
页数:11
相关论文
共 110 条
[1]   Commensal Escherichia coli of healthy humans: a reservoir for antibiotic-resistance determinants [J].
Bailey, Jannine K. ;
Pinyon, Jeremy L. ;
Anantham, Sashindran ;
Hall, Ruth M. .
JOURNAL OF MEDICAL MICROBIOLOGY, 2010, 59 (11) :1331-1339
[2]   Co-selection of antibiotic and metal resistance [J].
Baker-Austin, C ;
Wright, MS ;
Stepanauskas, R ;
McArthur, JV .
TRENDS IN MICROBIOLOGY, 2006, 14 (04) :176-182
[3]   Efficient removal of antibiotics in surface-flow constructed wetlands, with no observed impact on antibiotic resistance genes [J].
Berglund, Bjorn ;
Khan, Ghazanfar Ali ;
Weisner, Stefan E. B. ;
Ehde, Per Magnus ;
Fick, Jerker ;
Lindgren, Per-Eric .
SCIENCE OF THE TOTAL ENVIRONMENT, 2014, 476 :29-37
[4]   Diverse aadA gene cassettes on class 1 integrons introduced into soil via spread manure [J].
Binh, Chu T. T. ;
Heuer, Holger ;
Kaupenjohann, Martin ;
Smalla, Kornelia .
RESEARCH IN MICROBIOLOGY, 2009, 160 (06) :427-433
[5]   Comparative study of class 1 integron and Vibrio cholerae superintegron integrase activities [J].
Biskri, L ;
Bouvier, M ;
Guérout, AM ;
Boisnard, S ;
Mazel, D .
JOURNAL OF BACTERIOLOGY, 2005, 187 (05) :1740-1750
[6]   Integrons: mobilizable platforms that promote genetic diversity in bacteria [J].
Boucher, Yan ;
Labbate, Maurizio ;
Koenig, Jeremy E. ;
Stokes, H. W. .
TRENDS IN MICROBIOLOGY, 2007, 15 (07) :301-309
[7]   Local Mobile Gene Pools Rapidly Cross Species Boundaries To Create Endemicity within Global Vibrio cholerae Populations [J].
Boucher, Yan ;
Cordero, Otto X. ;
Takemura, Alison ;
Hunt, Dana E. ;
Schliep, Klaus ;
Bapteste, Eric ;
Lopez, Philippe ;
Tarr, Cheryl L. ;
Polz, Martin F. .
MBIO, 2011, 2 (02)
[8]   The integrons In0, In2, and In5 are defective transposon derivatives [J].
Brown, HJ ;
Stokes, HW ;
Hall, RM .
JOURNAL OF BACTERIOLOGY, 1996, 178 (15) :4429-4437
[9]   Fate of Antibiotic Resistance Genes and Class 1 Integrons in Soil Microcosms Following the Application of Treated Residual Municipal Wastewater Solids [J].
Burch, Tucker R. ;
Sadowsky, Michael J. ;
LaPara, Timothy M. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2014, 48 (10) :5620-5627
[10]   Aerobic digestion reduces the quantity of antibiotic resistance genes in residual municipal wastewater solids [J].
Burch, Tucker R. ;
Sadowsky, Michael J. ;
LaPara, Timothy M. .
FRONTIERS IN MICROBIOLOGY, 2013, 4