Construction of a kiwifruit yeast two-hybrid cDNA library to identify host targets of the Pseudomonas syringae pv. actinidiae effector AvrPto5

被引：8
作者：
Dharmaraj, Karthikeyan [1 ,2 ,3 ]
Cui, Wei [2 ]
Rikkerink, Erik H. A. [2 ]
Templeton, Matthew D. [1 ,2 ]
机构：
[1] Univ Auckland, Sch Biol Sci, Private Bag 92019, Auckland 1142, New Zealand
[2] New Zealand Inst Plant & Food Res Ltd, Private Bag 92169, Auckland 1142, New Zealand
[3] Minist Primary Ind, Plant Hlth & Environm Lab, 231 Morrin Rd, Auckland 1072, New Zealand
来源：
BMC RESEARCH NOTES | 2019年 / 12卷 / 1期
关键词：
Bacterial canker; cDNA library; Heavy metal-associated protein; Host target; Type three secreted effector;
D O I：
10.1186/s13104-019-4102-x
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Objective: Bacterial canker is a destructive disease of kiwifruit caused by the Gram-negative bacterium Pseudomonas syringae pv. actinidiae (Psa). To understand the disease-causing mechanism of Psa, a kiwifruit yeast two-hybrid cDNA library was constructed to identify putative host targets of the Psa Type Three Secreted Effector AvrPto5. Results: In this study, we used the Mate & Plate (TM) yeast two-hybrid library method for constructing a kiwifruit cDNA library from messenger RNA of young leaves. The constructed library consisted of 2.15 x 10(6) independent clones with an average insert size of 1.52 kb. The screening of the kiwifruit yeast two-hybrid cDNA library with Psa AvrPto5 revealed the interaction of a V-type proton ATPase subunit-H, a proline rich-protein and heavy metal-associated isoprenylated plant protein 26. Among these, heavy metal-associated isoprenylated plant protein 26 showed a positive interaction with Psa AvrPto5 as both prey and bait.
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页数：6
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