Comparative Proteomic Analysis of Extracellular Vesicles Isolated by Acoustic Trapping or Differential Centrifugation

被引：43

作者：

Rezeli, Melinda ^{[1
]}

Gidlof, Olof ^{[2
]}

Evander, Mikael ^{[1
]}

Bryl-Gorecka, Paulina ^{[2
]}

Sathanoori, Ramasri ^{[2
]}

Gilje, Patrik ^{[2
]}

Pawlowski, Krzysztof ^{[4
]}

Horvatovich, Peter ^{[5
]}

Erlinge, David ^{[2
]}

Marko-Varga, Gyorgy ^{[1
,3
]}

Laurell, Thomas ^{[1
,3
,6
]}

机构：

[1] Lund Univ, Dept Biomed Engn, Lund, Sweden

[2] Lund Univ, Dept Cardiol, Clin Sci, Lund, Sweden

[3] Lund Univ, Ctr Excellence Biol & Med Mass Spectrometry, Lund, Sweden

[4] Warsaw Univ Life Sci, Fac Agr & Biol, Dept Expt Design & Bioinformat, Warsaw, Poland

[5] Univ Groningen, Dept Pharm, Analyt Biochem, Groningen, Netherlands

[6] Dongguk Univ, Dept Biomed Engn, Seoul, South Korea

来源：

ANALYTICAL CHEMISTRY | 2016年 / 88卷 / 17期

基金：

瑞典研究理事会;

关键词：

PLATELET-DERIVED MICROPARTICLES; ELEVATION MYOCARDIAL-INFARCTION; CIRCULATING MICROPARTICLES; ENDOTHELIAL MICROPARTICLES; GENE-FUNCTION; BLOOD-PLASMA; DISEASE; MICROVESICLES; EXOSOMES; CELLS;

D O I：

10.1021/acs.analchem.6b01694

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Extracellular vesicles (ECVs), including micro particles and exosomes, are submicrometer membrane vesicles released by diverse cell types upon activation or stress. Circulating ECVs are potential reservoirs of disease biomarkers, and the complexity of these vesicles is significantly lower compared to their source, blood plasma, which makes ECV-based biomarker studies more promising. Proteomic profiling of ECVs is important not only to discover new diagnostic or prognostic markers but also to understand their roles in biological function. In the current study, we investigated the protein composition of plasma-derived ECVs isolated by acoustic seed trapping. Additionally, the protein composition of ECVs isolated with acoustic trapping was compared to that isolated with a conventional differential centrifugation protocol. Finally, the proteome of ECVs originating from ST-elevation myocardial infarction patients was compared with that of healthy controls using label-free LC-MS quantification. The acoustic trapping platform allows rapid and automated preparation of ECVs from small sample volumes, which are therefore well-suited for biobank repositories. We found that the protein composition of trapped ECVs is very similar to that isolated by the conventional differential centrifugation method.

引用

页码：8577 / 8586

页数：10

共 45 条

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