Separation and purification of furanocoumarins from Toddalia asiatica (L.) Lam. using microwave-assisted extraction coupled with high-speed counter-current chromatography

被引:30
作者
Qiu, Heyuan [2 ]
Xiao, Xiaohua [1 ]
Li, Gongke [1 ]
机构
[1] Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Guangdong, Peoples R China
[2] Hanshan Normal Univ, Dept Chem, Chaozhou, Peoples R China
基金
中国国家自然科学基金;
关键词
Furanocoumarin; High-speed counter-current chromatography; Microwave-assisted extraction; Toddalia asiatica (L; ) Lam; CHEMICAL-CONSTITUENTS; CNIDIUM-MONNIERI; MEDICINAL-PLANT; COUMARINS; ISOPIMPINELLIN;
D O I
10.1002/jssc.201100995
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A method of microwave-assisted extraction coupled with high-speed counter-current chromatography was established for separation and purification of isopimpinellin, pimpinellin and phellopterin from Toddalia asiatica (L.) Lam. The conditions of MAE including the extraction solvent, size of sample, solid/liquid ratio, extraction temperature and extraction time were optimized by a mono-factor test. That is, 2.0 g dried powder of T. asiatica (L.) Lam of 0.30-0.15 mm size was extracted with 20 mL (solid/liquid ratio of 1:10, g/mL) methanol under 50 degrees C for 1 min. The crude extract was separated and purified by high-speed counter-current chromatography with hexaneethyl acetatemethanolwater (5:5:5.5:4.5, v/v/v/v) solvent system. 0.85 mg/g of isopimpinellin, 2.55 mg/g of pimpinellin and 0.95 mg/g of phellopterin were obtained from original sample in one-step within 240 min, the purity determined by high performance liquid chromatography was 95.0%, 99.1% and 96.4%, respectively. Their chemical structures were further identified by mass spectroscopy and nuclear magnetic resonance spectroscopy. The results demonstrated that microwave-assisted extraction coupled with high-speed counter-current chromatography was a feasible, economical and efficient technique for rapid extraction, separation and purification of effective compounds from natural products.
引用
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页码:901 / 906
页数:6
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