Ucma is not necessary for normal development of the mouse skeleton

被引:32
作者
Eitzinger, Nicole [1 ,3 ]
Surmann-Schmitt, Cordula [1 ]
Boesl, Michael [2 ]
Schett, Georg [3 ]
Engelke, Klaus [4 ]
Hess, Andreas [5 ]
von der Mark, Klaus [1 ]
Stock, Michael [1 ,3 ]
机构
[1] Univ Erlangen Nurnberg, Sch Med, Dept Expt Med 1, Nikolaus Fiebiger Ctr Mol Med, D-91054 Erlangen, Germany
[2] Max Planck Inst Neurobiol, D-82152 Martinsried, Germany
[3] Univ Erlangen Nurnberg, Erlangen Med Sch, Dept Internal Med 3, D-91054 Erlangen, Germany
[4] Univ Erlangen Nurnberg, Inst Med Phys, D-91054 Erlangen, Germany
[5] Univ Erlangen Nurnberg, Inst Pharmacol & Toxicol, D-91054 Erlangen, Germany
关键词
Ucma; Knockout; Gla; Skeletal development; Gene expression; MICE LACKING; VITAMIN-K; EXTRACELLULAR-MATRIX; CARTILAGE; PROTEIN; EXPRESSION; GENE; MATRILIN-3;
D O I
10.1016/j.bone.2011.11.017
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Ucma (Upper zone of growth plate and Cartilage Matrix Associated protein) is a highly conserved tyrosine-sulphated secreted protein of Mw 17 kDa, which is expressed by juvenile chondrocytes. To evaluate the physiological function of this novel cartilage protein, we generated a Ucma-deficient mouse strain by introducing a lacZ/neoR-cassette into the first exon of the Ucma gene. This mutation results in the complete loss of Ucma mRNA and protein expression. Surprisingly, however, although previous in vitro studies implied a role for Ucma in calcification and ossification, these processes were not affected in Ucma-deficient mice during normal development. Likewise, cartilage development was normal. While in previous works Ucma was mainly detected in the cartilage of embryonic and young mice, we detected Ucma expression also in the adult cartilage of the ribs using the lacZ cassette under the control of the Ucma promoter. Moreover, Ucma protein was specifically detected in adult growth plate cartilage by immunohistochemistry. Considering that skeletal development in Ucma-deficient mice is not significantly impaired, protein expression in adult cartilage indicates that Ucma might be involved in skeletal homeostasis and in the mechanical properties of the skeleton during challenging conditions such as ageing or disease. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:670 / 680
页数:11
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