Efficient amplification of insert end sequences from bacterial artificial chromosome clones by thermal asymmetric interlaced PCR

被引：139
作者：
Liu, YG
Huang, N
机构：
[1] S China Agr Univ, Ctr Biotechnol, Guangzhou 510642, Peoples R China
[2] Int Rice Res Inst, Manila 1099, Philippines
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 1998年 / 16卷 / 02期
关键词：
bacterial artificial chromosome; chromosome walking; insert end sequences;
D O I：
10.1023/A:1007420918645
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
TAIL-PCR is a powerful tool for the recovery of DNA fragments adjacent to known sequences. A protocol is presented for the amplification of insert end sequences from bacterial artificial chromosome clones using TAIL-PCR. The amplified products are suitable as probes for chromosome walking and genome mapping and as templates for direct sequencing. The protocol has been used in rice genome studies.
引用
收藏
页码：175 / 181
页数：7
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