Effects of a non-ionic surfactant, Tween 20, on adsorption/desorption of saccharification enzymes onto/from lignocelluloses and saccharification rate

被引：82

作者：

Seo, Dong-June ^{[1
]}

Fujita, Hirotaka ^{[1
]}

Sakoda, Akiyoshi ^{[1
]}

机构：

[1] Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan

来源：

ADSORPTION-JOURNAL OF THE INTERNATIONAL ADSORPTION SOCIETY | 2011年 / 17卷 / 05期

关键词：

Lignocelluloses; Tween; 20; Cellulase; beta-glucosidase; ENZYMATIC-HYDROLYSIS; POLY(ETHYLENE GLYCOL); CELLULASE; ADSORPTION; BIOMASS; SOFTWOOD; LIGNIN; DEACTIVATION;

D O I：

10.1007/s10450-011-9340-8

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this work, we examined the role of a non-ionic surfactant, Tween 20, on enzymatic hydrolysis of lignocelluloses. Delignified lignocelluloses (pine wood chip) were used as model substrates. Effects of Tween 20 on adsorption/desorption onto/from lignocelluloses with and without hydrolysis were evaluated respectively. Tween 20 lowered the non-biospecific adsorption of beta-glucosidase and enhanced the bio-specific adsorption of cellulase. Tween 20 did not affect the liquid phase reaction (cellobiose hydrolysis). However, for the solid surface reaction (cellulose hydrolysis), cellulose conversion for 72 hrs was increased 9-21% and 1-8.5% for samples with high lignin contents (PI) and low lignin contents (PIII) by injection of Tween 20 (0.024-0.24 mM), respectively. Moreover, Tween 20 increased the cellulose conversion rate substantially. It is suggested that the increase of cellulase amount adsorbed due to the increase of effective cellulose surface by Tween 20 contribute to the enhancement of cellulose conversion.

引用

页码：813 / 822

页数：10

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