Role of Signal Transducer and Activator of Transcription-3 in Up-Regulation of GFAP After Epilepsy

被引：62

作者：

Xu, Zucai ^{[1
,2
]}

Xue, Tao ^{[1
]}

Zhang, Zuxia ^{[1
]}

Wang, Xuefeng ^{[2
]}

Xu, Ping ^{[3
]}

Zhang, Jun ^{[3
]}

Lei, Xianze ^{[3
]}

Li, Yuqin ^{[1
]}

Xie, Yunlan ^{[1
]}

Wang, Liang ^{[2
]}

Fang, Min ^{[2
]}

Chen, Yangmei ^{[1
]}

机构：

[1] Chongqing Med Univ, Affiliated Hosp 2, Dept Neurol, Chongqing 400010, Peoples R China

[2] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurol, Chongqing 400016, Peoples R China

[3] Zunyi Med Coll, Dept Neurol, Affiliated Hosp, Zunyi Guizhou 563003, Peoples R China

来源：

NEUROCHEMICAL RESEARCH | 2011年 / 36卷 / 12期

关键词：

Epileptogenesis; Astrogliosis; p-STAT3; GFAP; TEMPORAL-LOBE EPILEPSY; JAK-STAT PATHWAY; EPILEPTOGENESIS; HIPPOCAMPUS; ASTROCYTES; DYSFUNCTION; ISCHEMIA; SEIZURES; GLIOSIS; CELLS;

D O I：

10.1007/s11064-011-0576-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glial Fibrillary Acidic Protein (GFAP) is regarded as a marker of reactive astrogliosis. Recent studies have demonstrated that signal transducer and activator of transcription-3, STAT3 regulates GFAP expression after brain injuries. However, whether STAT3 controls astrogliosis in epilepsy is not clear. In this study, we measured p-STAT3 and GFAP expression during the epileptic process using immunohistochemistry, Western blotting and immunofluorescence. Both p-STAT3 and GFAP expression were highly expressed in the rat hippocampus during different phases of the epileptic process. The augmentation of GFAP expression was inhibited by AG490, a janus kinase 2 (JAK2, an upstream gene of STAT3) inhibitor. The coexpression of p-STAT3 and GFAP was detected in the epileptic rat hippocampus and temporal neocortex of patients. These findings indicate that epilepsy involves the activation of STAT3 that up-regulates the expression of GFAP, which may play an important role in epileptogenesis.

引用

页码：2208 / 2215

页数：8

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