FTIR study on the normal and cancerous stomach tissues

Tissues of cancerous and corresponding normal stomach were studied by FTIR technique. The results showed that there are obvious differences between FTIR spectra of them in spectral parameters such as frequency, intensity and band shape etc. The changes involving the phosphate symmetric stretching v(s,PO2)(-) and asymmetric stretching v(as,PO2)(-) modes, the CH(3) and CH(2) groups stretching (v(s,CH2), v(as,CH3)) and bending (delta (CH2)) modes and the C-O stretching v(C-O) mode were discussed. In addition, the changes of structure of hydrogen-bonding of nucleic acid and cell proteins and the packing and the conformational structure of the membrane lipids were analysed further. The average wavenumber of band of v(s,PO2)(-) shifted from 1 080.92 cm(-1) to 1 085.93 cm(-1) and that of v(as,PO2)(-).,pc shifted from 1 239.64 cm(-1) to 1 238.73 cm(-1) which indicated that the degree of hydrogen-bonding formed by oxygen atom of the phosphodiester groups of nucleic acids was increased. The average wavenumber of band of delta (CH2) of membrane lipids shifted from 1 455.23 cm(-1) to 1 457.37 cm(-1) that suggested that the conformational structure of the methylene chains of membrane lipids is more disordered than in normal tissues. The shift of band of v(C-O) of cell proteins from 1 166.08 cm(-1) to 1 166.58 cm(-1) indicated that the hydrogen-bond of cell proteins become weaker.