Study of platelet-rich fibrin combined with rat periodontal ligament stem cells in periodontal tissue regeneration

被引：34

作者：

Duan, Xuejing ^{[1
]}

Lin, Zhiyong ^{[1
]}

Lin, Xiujuan ^{[2
]}

Wang, Zhiqiang ^{[1
]}

Wu, Yihua ^{[1
]}

Ji, Mei ^{[1
]}

Lu, Wei ^{[1
]}

Wang, Xiaoyang ^{[1
]}

Zhang, Dongsheng ^{[1
]}

机构：

[1] Shandong Univ, Sch Stomatol, Shandong Prov Hosp, Jinan, Shandong, Peoples R China

[2] Shandong Univ, Sch Stomatol, Qianfoshan Hosp, Jinan, Shandong, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2018年 / 22卷 / 02期

基金：

中国国家自然科学基金;

关键词：

periodontal regeneration; platelet-rich fibrin; periodontal ligament stem cells; CLINICAL-TRIAL; PROLIFERATION; DEFECTS; BONE; PLASMA; DIFFERENTIATION; OSTEOBLASTS; EXPRESSION;

D O I：

10.1111/jcmm.13461

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The objective of this study was to investigate the advantages and feasibility of periodontal tissue regeneration using platelet-rich fibrin (PRF) combined with rat periodontal ligament stem cells (PDLSCs) for the first time. We first determined the effect of PRF on rat PDLSCs invitro. We next conducted an invivo study, in which a tissue engineering technique was performed to repair periodontal defects in five groups: a blank group, collagen group (implanted collagen membrane), collagen+cells group (implanted collagen membrane and rat PDLSCs), PRF group (implanted PRF membrane) and PRF+cells group (implanted PRF membrane and rat PDLSCs). PRF greatly enhanced cell proliferation, mRNA and protein expression levels of bone sialoprotein (BSP), osteocalcin (OC), and runt-related transcription factor 2 (RUNX2) and activity of alkaline phosphatase (ALP) invitro. Transplantation of PRF combined with rat PDLSCs resulted in higher expression of osteopontin (Opn), collagen I (COL1A) and RUNX2 at both 12 and 24days after surgery. Micro-computed tomography and histological analysis showed substantially more new bone formation in the PRF+cells group at 24days after surgery. Based on these results, we discuss the role of PRF in the proliferation and differentiation of rat PDLSCs and suggest that PRF combined with rat PDLSCs provides a valuable tool for periodontal tissue engineering.

引用

页码：1047 / 1055

页数：9

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