Protective efficacy of recombinant Yersinia outer proteins against bubonic plague caused by encapsulated and nonencapsulated Yersinia pestis

被引：60

作者：

Andrews, GP ^{[1
]}

Strachan, ST ^{[1
]}

Benner, GE ^{[1
]}

Sample, AK ^{[1
]}

Anderson, GW ^{[1
]}

Adamovicz, JJ ^{[1
]}

Welkos, SL ^{[1
]}

Pullen, JK ^{[1
]}

Friedlander, AM ^{[1
]}

机构：

[1] USA, Med Res Inst Infect Dis, Div Bacteriol, Ft Detrick, MD 21702 USA

来源：

INFECTION AND IMMUNITY | 1999年 / 67卷 / 03期

关键词：

D O I：

10.1128/IAI.67.3.1533-1537.1999

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To evaluate the role of Yersinia outer proteins (Yops) in conferring protective immunity against plague, six yap loci from Yersinia pestis were individually amplified by PCR, cloned, and expressed in Escherichia coli. The recombinant proteins were purified and injected into mice. Most Yop-vaccinated animals succumbed to infection with either wild-type encapsulated Y. pestis or a virulent, nonencapsulated isogenic variant. Vaccination with YpkA significantly prolonged mean survival time but did not increase overall survival of mice infected with the nonencapsulated strain. The only significant protection against death was observed in YopD-vaccinated mice challenged with the nonencapsulated strain.

引用

页码：1533 / 1537

页数：5

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