In silico and in vitro studies of Taiwan Chingguan Yihau (NRICM101) on TNF-α/IL-1β-induced Human Lung Cells

被引：12

作者：

Cheng, Yih-Dih ^{[1
,2
]}

Lu, Chi-Cheng ^{[3
]}

Hsu, Yuan-Man ^{[4
,5
]}

Tsai, Fuu-Jen ^{[6
,7
,8
]}

Bau, Da-Tian ^{[9
,10
,11
]}

Tsai, Shih-Chang ^{[12
]}

Cheng, Ching-Chang ^{[13
]}

Lin, Jen-Jyh ^{[14
]}

Huang, Yuang-Yu ^{[15
]}

Juan, Yu-Ning ^{[15
]}

Chiu, Yu-Jen ^{[16
,17
,18
]}

Kuo, Sheng-Chu ^{[1
]}

Yang, Jai-Sing ^{[15
]}

Wu, Lii-Tzu ^{[19
]}

机构：

[1] China Med Univ, Sch Pharm, Taichung 406040, Taiwan

[2] China Med Univ Hosp, Dept Pharm, Taichung 404327, Taiwan

[3] Natl Taiwan Univ Sport, Dept Sport Performance, Taichung 404401, Taiwan

[4] China Med Univ, Coll Life Sci, Dept Biol Sci & Technol, Taichung 406040, Taiwan

[5] Tunghai Univ, Agr Coll, Dept Anim Sci & Biotechnol, Taichung 407224, Taiwan

[6] China Med Univ, Coll Chinese Med, Sch Chinese Med, Taichung 404333, Taiwan

[7] China Med Univ, Childrens Hosp, Taichung 404327, Taiwan

[8] China Med Univ Hosp, Dept Med Genet, Taichung 404327, Taiwan

[9] China Med Univ, Grad Inst Biomed Sci, Taichung 404333, Taiwan

[10] China Med Univ Hosp, Dept Med Res, Terry Fox Canc Res Lab, Taichung 404327, Taiwan

[11] Asia Univ, Dept Bioinformat & Med Engn, Taichung 413305, Taiwan

[12] China Med Univ, Dept Biol Sci & Technol, Taichung 406040, Taiwan

[13] China Med Univ, Lab Anim Serv Ctr, Taichung 404333, Taiwan

[14] China Med Univ Hosp, Dept Med, Div Cardiol, Taichung 404327, Taiwan

[15] China Med Univ, China Med Univ Hosp, Dept Med Res, Taichung 404327, Taiwan

[16] Taipei Vet Gen Hosp, Dept Surg, Div Plast & Reconstruct Surg, Taipei 112201, Taiwan

[17] Natl Yang Ming Chiao Tung Univ, Sch Med, Dept Surg, Taipei 112304, Taiwan

[18] Natl Yang Ming Chiao Tung Univ, Inst Clin Med, Taipei 112304, Taiwan

[19] China Med Univ, Sch Med, Dept Microbiol, 91 Hsueh Shih Rd, Taichung 404333, Taiwan

来源：

BIOMEDICINE-TAIWAN | 2022年 / 12卷 / 03期

关键词：

Taiwan chingguan Yihau (NRICM101); Traditional Chinese medicine (TCM); TNF-alpha/IL-1 beta-induced lung cell injury; High throughput target screening platform; Next-generation sequencing (NGS); COVID-19; INFLAMMATION; INVOLVEMENT;

D O I：

10.37796/2211-8039.1378

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

COVID-19 pandemic has been a global outbreak of coronavirus (SARS-CoV-2 virus) since 2019. Taiwan Chingguan Yihau (NRICM101) is the first traditional Chinese medicine (TCM) classic herbal formula and is widely used for COVID-19 patients in Taiwan and more than 50 nations. This study is to investigate in silico target fishing for the components of NRICM101 and to explore whether NRICM101 inhibits cytokines-induced normal human lung cell injury in vitro. Our results showed that network prediction of NRICM101 by a high throughput target screening platform showed that NRICM101 has multiple functions that may affect cytokine regulation to prevent human lung cell injury. In addition, NRICM101 revealed protective effects against TNF-alpha/IL-1 beta-induced normal human lung HEL 299 cell injury through JNK and p38MAPK kinase signaling. Next-generation sequencing (NGS) analysis of NRICM101 on TNF-alpha/IL-1 beta-injured HEL 299 cells indicated that inflammatory pathway, cell movement of macrophages, cellular infiltration by macrophages, and Th1/Th2 immuno-regulation pathways were included. Thus, NRICM101 is a therapeutic agent, and it can improve COVID-19 syndrome to confer beneficial effects through multiple targeting and multiple mechanisms.

引用

页码：56 / 71

页数：17

共 29 条

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