Synthesis of Poly(propylene glycol)-block-Polyethylenimine Triblock Copolymers for the Delivery of Nucleic Acids

被引：10

作者：

Brissault, Blandine ^{[2
]}

Leborgne, Christian ^{[1
]}

Scherman, Daniel ^{[3
]}

Guis, Christine ^{[4
]}

Kichler, Antoine ^{[1
,3
]}

机构：

[1] Genethon, F-91002 Evry, France

[2] Equipe Syst Polymeres Complexes, Inst Chim & Mat Paris Est, UMR 7182, F-94320 Thiais, France

[3] Univ Paris 05, CNRS, INSERM, UMR 8151,U1022, Paris, France

[4] Univ Evry, Lab Anal & Modelisat Biol & Environn, UMR 8587, CNRS CEA UEVE, F-91025 Evry, France

来源：

MACROMOLECULAR BIOSCIENCE | 2011年 / 11卷 / 05期

关键词：

amphiphiles; block copolymers; drug delivery systems; self-assembly; small interfering RNA; LINEAR POLYETHYLENIMINE; RNA INTERFERENCE; SIRNA DELIVERY; IN-VIVO; MAMMALIAN-CELLS; GENE DELIVERY; BEHAVIOR; DERIVATIVES; VITRO;

D O I：

10.1002/mabi.201000404

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

LPEIs, which are efficient DNA transfection agents, were found to be far less effective for the delivery of siRNAs. Here, two amphiphilic triblock copolymers LPEI(50)-b-PPG(36)-b-LPEI(50) (2) and LPEI(14)-b-PPG(68)-b-LPEI(14) (4) have been synthesized. The transfection assays showed that compound 2 was efficient for DNA transfection whilst it was almost inactive for siRNA delivery. In contrast, polymer 4 was inefficient for DNA transfection while it showed capabilities for siRNA delivery. Taken together, our results indicate that the properties required for DNA and siRNA delivery are different. Moreover, we show that introduction of a hydrophobic segment that allows self-assembly confers siRNA delivery capacities.

引用

页码：652 / 661

页数：10

共 24 条

[1] Silence of the transcripts: RNA interference in medicine [J].

Barik, S .

JOURNAL OF MOLECULAR MEDICINE-JMM, 2005, 83 (10) :764-773

[2] Sticky overhangs enhance siRNA-mediated gene silencing [J].

Bolcato-Bellemin, Anne-Laure ;

Bonnet, Marie-Elise ;

Creusatt, Gaeelle ;

Erbacher, Patrick ;

Behr, Jean-Paul .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (41) :16050-16055

[3] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE [J].

BOUSSIF, O ;

LEZOUALCH, F ;

ZANTA, MA ;

MERGNY, MD ;

SCHERMAN, D ;

DEMENEIX, B ;

BEHR, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7297-7301

[4] Linear topology confers in vivo gene transfer activity to polyethylenimines [J].

Brissault, B. ;

Leborgne, C. ;

Guis, C. ;

Danos, O. ;

Cheradame, H. ;

Kichler, A. .

BIOCONJUGATE CHEMISTRY, 2006, 17 (03) :759-765

[5] Kinetic study of poly(ethylene oxide-b-2-methyl-2-oxazoline) diblocs synthesis from poly(ethylene oxide) macroinitiators [J].

Brissault, B ;

Guis, C ;

Cheradame, H .

EUROPEAN POLYMER JOURNAL, 2002, 38 (02) :219-228

[6] Synthesis of linear polyethylenimine derivatives for DNA transfection [J].

Brissault, B ;

Kichler, A ;

Guis, C ;

Leborgne, C ;

Danos, O ;

Cheradame, H .

BIOCONJUGATE CHEMISTRY, 2003, 14 (03) :581-587

[7] Amphiphilic poly[(propylene glycol)-block-(2methyl-2-oxazoline)] copolymers for gene transfer in skeletal muscle [J].

Brissault, Blandine ;

Kichler, Antoine ;

Leborgne, Christian ;

Jarroux, Nathalie ;

Cheradame, Herv ;

Guis, Christine .

CHEMMEDCHEM, 2007, 2 (08) :1202-1207

[8] Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems [J].

Caplen, NJ ;

Parrish, S ;

Imani, F ;

Fire, A ;

Morgan, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9742-9747

[9] Self-Assembling Polyethylenimine Derivatives Mediate Efficient siRNA Delivery in Mammalian Cells [J].

Creusat, Gaelle ;

Zuber, Guy .

CHEMBIOCHEM, 2008, 9 (17) :2787-2789

[10] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].

Elbashir, SM ;

Harborth, J ;

Lendeckel, W ;

Yalcin, A ;

Weber, K ;

Tuschl, T .

NATURE, 2001, 411 (6836) :494-498

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