Electrochemical enzyme immunoassay using model labels

被引:78
作者
Li, Xue-Mei [1 ]
Yang, Xiao-Yan [1 ]
Zhang, Shu-Sheng [1 ]
机构
[1] Qingdao Univ Sci & Technol, Key Lab Ecochem Engn, Minist Educ, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China
基金
中国国家自然科学基金;
关键词
alkaline phosphatase; ALP; EIA; electrochemical detection; enzyme immunoassay; enzyme label; glucose oxidase; GOx; horseradish peroxidase; HRP;
D O I
10.1016/j.trac.2008.04.002
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Enzyme immunoassays (EIAs) based on electrochemical detection offer several potential advantages and have been applied in clinical, medical, biotechnological, food and environmental analysis. Among the enzyme labels employed, horseradish peroxidase (HRP), alkaline phosphatase (ALP) and glucose oxidase (GOx) are the most common. This brief review reflects recent advances, challenges, and trends of electrochemical EIAs focusing on HRP, ALP or GOx as labels over the past five years. We especially emphasize current development of EIAs combined with other developments, including nanotechnology and miniaturization. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:543 / 553
页数:11
相关论文
共 79 条
[1]   Self-contained microelectrochemical immunoassay for small volumes using mouse IgG as a model system [J].
Aguilar, ZP ;
Vandaveer, WR ;
Fritsch, I .
ANALYTICAL CHEMISTRY, 2002, 74 (14) :3321-3329
[2]   Biomedical applications of electrostatic layer-by-layer nano-assembly of polymers, enzymes, and nanoparticles [J].
Ai, H ;
Jones, SA ;
Lvov, YM .
CELL BIOCHEMISTRY AND BIOPHYSICS, 2003, 39 (01) :23-43
[3]   3D protein microarrays:: Performing multiplex immunoassays on a single chip [J].
Angenendt, P ;
Glökler, J ;
Konthur, Z ;
Lehrach, H ;
Cahill, DJ .
ANALYTICAL CHEMISTRY, 2003, 75 (17) :4368-4372
[4]   Sensitive estimation of total cholesterol in blood using Au nanowires based micro-fluidic platform [J].
Aravamudhan, Shyam ;
Kumar, Arun ;
Mohapatra, Shyam ;
Bhansali, Shekhar .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (9-10) :2289-2294
[5]   Immunoassay with multicomponent protein microarrays fabricated by electrospray deposition [J].
Avseenko, NV ;
Morozova, TY ;
Ataullakhanov, FI ;
Morozov, VN .
ANALYTICAL CHEMISTRY, 2002, 74 (05) :927-933
[6]   Electrochemical determination of carbohydrates: Enzyme electrodes and amperometric detection in liquid chromatography and capillary electrophoresis [J].
Baldwin, RP .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1999, 19 (1-2) :69-81
[7]   Micromosaic immunoassays [J].
Bernard, A ;
Michel, B ;
Delamarche, E .
ANALYTICAL CHEMISTRY, 2001, 73 (01) :8-12
[8]  
Bobes CF, 2001, ELECTROANAL, V13, P559, DOI 10.1002/1521-4109(200105)13:7<559::AID-ELAN559>3.0.CO
[9]  
2-6
[10]   DNA-directed protein immobilization for simultaneous detection of multiple analytes by surface plasmon resonance biosensor [J].
Boozer, C ;
Ladd, J ;
Chen, SF ;
Jiang, ST .
ANALYTICAL CHEMISTRY, 2006, 78 (05) :1515-1519