klavier (klv), A novel hypernodulation mutant of Lotus japonicus affected in vascular tissue organization and floral induction

被引:90
作者
Oka-Kira, E
Tateno, K
Miura, K
Haga, T
Hayashi, M
Harada, K
Sato, S
Tabata, S
Shikazono, N
Tanaka, A
Watanabe, Y
Fukuhara, I
Nagata, T
Kawaguchi, M
机构
[1] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, Tokyo 1130033, Japan
[2] Japan Sci & Technol Agcy, CREST, Kawaguchi, Saitama 3320012, Japan
[3] Gakushuin Univ, Fac Sci, Inst Biomol Sci, Toshima Ku, Tokyo 1718588, Japan
[4] Chiba Univ, Fac Hort, Chiba 2718510, Japan
[5] Kazusa DNA Res Inst, Chiba 2920818, Japan
[6] JAERI, Dept Radiat Res Environm & Resources, Gunma 3701292, Japan
[7] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, Tokyo 1538902, Japan
关键词
Lotus japonicus; hypernodulation; legume-Rhizobium symbiosis; autoregulation; vascular tissue; late flowering;
D O I
10.1111/j.1365-313X.2005.02543.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A novel hypernodulation mutant line was isolated from Lotus japonicus Miyakojima MG-20 by irradiation with a helium ion beam. This mutant, named klavier (klv), had roots that were densely covered with small nodules. The nodulation zone of klv was significantly wider than that of the wild type. Grafting experiments showed that klv is impaired in the long-distance shoot-to-root autoregulatory mechanism. Thus the shoot genotype was found to be responsible for the negative regulation of nodule development by KLV. Nodulation of klv showed a higher tolerance to nitrogen (KNO3) than the wild type, which is a common feature of hypernodulating mutants. In addition to an increased number of nodules, the klv mutant showed convex leaf veins on the adaxial leaf surface, markedly delayed flowering and dwarf phenotypes. Microscopic examination of the leaf veins revealed that they were discontinuous. Other phenotypes such as fasciated stems, increased number of flowers and bifurcated pistils were also frequently observed in the klv mutant. Among these phenotypes, hypernodulation, aberrant leaf vein formation and significantly delayed flowering were all linked in a monogenic and recessive manner, indicating that these phenotypes are caused by either a single mutation, or tightly linked mutations. KLV was mapped within 0.29 cM on the long arm of chromosome 1.
引用
收藏
页码:505 / 515
页数:11
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