Multivariate optical computing using a digital micromirror device for fluorescence and Raman spectroscopy

被引：19

作者：

Smith, Zachary J. ^{[1
]}

Strombom, Sven ^{[1
]}

Wachsmann-Hogiu, Sebastian ^{[1
,2
]}

机构：

[1] Univ Calif Davis, Ctr Biophoton Sci & Technol, Sacramento, CA 95817 USA

[2] Univ Calif Davis, Dept Pathol & Lab Med, Sacramento, CA 95817 USA

来源：

OPTICS EXPRESS | 2011年 / 19卷 / 18期

基金：

美国国家科学基金会;

关键词：

PREDICTIVE SPECTROSCOPY; CELLS; IDENTIFICATION; MICROSCOPY; BACTERIA; ELEMENT;

D O I：

10.1364/OE.19.016950

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A multivariate optical computer has been constructed consisting of a spectrograph, digital micromirror device, and photomultiplier tube that is capable of determining absolute concentrations of individual components of a multivariate spectral model. We present experimental results on ternary mixtures, showing accurate quantification of chemical concentrations based on integrated intensities of fluorescence and Raman spectra measured with a single point detector. We additionally show in simulation that point measurements based on principal component spectra retain the ability to classify cancerous from noncancerous T cells. (C) 2011 Optical Society of America

引用

页码：16950 / 16962

页数：13

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