Watching proteases in action

被引：11

作者：

Brix, K ^{[1
]}

Jordans, S ^{[1
]}

机构：

[1] Int Jacobs Univ Bremen, Sch Sci & Engn, D-28759 Bremen, Germany

来源：

NATURE CHEMICAL BIOLOGY | 2005年 / 1卷 / 04期

关键词：

D O I：

10.1038/nchembio0905-186

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Activity-based probes can be used for monitoring enzyme activity based on their covalent reactions with active-site residues. A quenched activity-based probe has now been developed that becomes fluorescent only after labeling active proteases. The specificity of the fluorescent signal and cell permeability of the small molecule make this probe effective for monitoring protease activity in living cells.

引用

页码：186 / 187

页数：3

共 11 条

[1] Enzyme activity - it's all about image [J].

Baruch, A ;

Jeffery, DA ;

Bogyo, M .

TRENDS IN CELL BIOLOGY, 2004, 14 (01) :29-35

[2] Dynamic imaging of protease activity with fluorescently quenched activity-based probes [J].

Blum, G ;

Mullins, SR ;

Keren, K ;

Fonovic, M ;

Jedeszko, C ;

Rice, MJ ;

Sloane, BF ;

Bogyo, M .

NATURE CHEMICAL BIOLOGY, 2005, 1 (04) :203-209

[3]

BRIX K, EUREKAH BIOSCIENCE D, pCH5

[4] Emerging roles for cysteine proteases in human biology [J].

Chapman, HA ;

Riese, RJ ;

Shi, GP .

ANNUAL REVIEW OF PHYSIOLOGY, 1997, 59 :63-88

[5] Thyroid functions of mouse cathepsins B, K, and L [J].

Friedrichs, B ;

Tepel, C ;

Reinheckel, T ;

Deussing, J ;

von Figura, K ;

Herzog, V ;

Peters, C ;

Saftig, P ;

Brix, K .

JOURNAL OF CLINICAL INVESTIGATION, 2003, 111 (11) :1733-1745

[6] Enzyme activity profiles of the secreted and membrane proteome that depict cancer cell invasiveness [J].

Jessani, N ;

Liu, YS ;

Humphrey, M ;

Cravatt, BF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (16) :10335-10340

[7] Trafficking of lysosomal cathepsin B-green fluorescent protein to the surface of thyroid epithelial cells involves the endosomal/lysosomal compartment [J].

Linke, M ;

Herzog, V ;

Brix, K .

JOURNAL OF CELL SCIENCE, 2002, 115 (24) :4877-4889

[8] MEROPS:: the protease database [J].

Rawlings, ND ;

O'Brien, E ;

Barrett, AJ .

NUCLEIC ACIDS RESEARCH, 2002, 30 (01) :343-346

[9] Towards specific functions of lysosomal cysteine peptidases: phenotypes of mice deficient for cathepsin B or cathepsin L [J].

Reinheckel, T ;

Deussing, J ;

Roth, W ;

Peters, C .

BIOLOGICAL CHEMISTRY, 2001, 382 (05) :735-741

[10] Cysteine proteases of parasitic organisms [J].

Sajid, M ;

McKerrow, JH .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2002, 120 (01) :1-21

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