Determination of Propofol Using High Performance Liquid Chromatography in Whole Blood with Fluorescence Detection

被引:26
作者
Yarbrough, Jason [1 ]
Harvey, Ralph [2 ]
Cox, Sherry [1 ]
机构
[1] Univ Tennessee, Dept Biomed & Diagnost Sci, Knoxville, TN 37996 USA
[2] Univ Tennessee, Coll Vet Med, Dept Small Anim Clin Sci, Knoxville, TN 37996 USA
关键词
SOLID-PHASE EXTRACTION; HUMAN PLASMA; SAMPLE PREPARATION; MASS-SPECTROMETRY; ASSAY; PHARMACOKINETICS; METABOLITES; INFUSION; SURGERY; URINE;
D O I
10.1093/chromsci/bmr042
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
High-performance liquid chromatography method for the determination of propofol has been developed and validated. Following a liquid extraction using ethyl acetate and hexane, samples were separated by reverse-phase high-performance liquid chromatography on an XBridge C18 column and quantified using fluorescence detection at an excitation of 276 nm and an emission of 310 nm. The mobile phase was a mixture of water (pH 4.0) and acetonitrile, with a flow rate of 1.5 mL/min. The standard curve ranged from 52000 ng/mL. Intra-and inter-assay variability for propofol was less than 10, and the average recovery was greater than 95. This assay is suitable for use in pharmacokinetic studies. © 2012 The Author.
引用
收藏
页码:162 / 166
页数:5
相关论文
共 28 条
  • [1] A new method for the quantitation of propofol in human plasma: efficient solid-phase extraction and liquid chromatography/APCI-triple quadrupole mass spectrometry detection
    Bajpai, L
    Varshney, M
    Seubert, CN
    Dennis, DM
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 810 (02): : 291 - 296
  • [2] Development of a rapid and sensitive LC-ESI/MS/MS assay for the quantification of propofol using a simple off-line dansyl chloride derivatization reaction to enhance signal intensity
    Beaudry, F
    Guénette, SA
    Winterborn, A
    Marier, JF
    Vachon, P
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2005, 39 (3-4) : 411 - 417
  • [3] Pharmacokinetic and pharmacodynamic evaluation of propofol administered to cats in a novel, aqueous, nano-droplet formulation or as an oil-in-water macroemulsion
    Cleale, R. M.
    Muir, W. W.
    Waselau, A. -C.
    Lehmann, M. W.
    Amodie, D. M.
    Lerche, P.
    [J]. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 2009, 32 (05) : 436 - 445
  • [4] Quantitative measurement of propofol and in main glucuroconjugate metabolites in human plasma using solid phase extraction-liquid chromatography-tandem mass spectrometry
    Cohen, S.
    Lhuillier, F.
    Mouloua, Y.
    Vignal, B.
    Favetta, P.
    Guitton, J.
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 854 (1-2): : 165 - 172
  • [5] Court MH, 1999, DRUG METAB DISPOS, V27, P1293
  • [6] Cuadrado A., 1998, FIND EXP CLIN PHARM, V20, P397
  • [7] A rapid and simple HPLC method for the analysis of propofol in biological fluids
    Cussonneau, Xavier
    De Smet, Els
    Lantsoght, Kristof
    Salvi, Jean-Paul
    Bolon-Larger, Magali
    Boulieu, Roselyne
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2007, 44 (03) : 680 - 682
  • [8] Dawidowicz AL, 2000, BIOMED CHROMATOGR, V14, P493, DOI 10.1002/1099-0801(200011)14:7<493::AID-BMC999>3.0.CO
  • [9] 2-T
  • [10] HPLC investigation of free and bound propofol in human plasma and cerebrospinal fluid
    Dawidowicz, AL
    Kalitynski, R
    [J]. BIOMEDICAL CHROMATOGRAPHY, 2003, 17 (07) : 447 - 452