β-ecdysterone from Cyanotis arachnoidea exerts hypoglycemic effects through activating IRS-1/Akt/GLUT4 and IRS-1/Akt/GLUT2 signal pathways in KK-Ay mice

被引：15

作者：

Chen, Li ^{[1
]}

Zheng, Sijian ^{[1
]}

Huang, Mi ^{[1
]}

Ma, Xinhua ^{[1
]}

Yang, Jie ^{[1
]}

Deng, Shihao ^{[1
]}

Huang, Yun ^{[1
]}

Wen, Yanzhang ^{[1
]}

Yang, Xinzhou ^{[1
]}

机构：

[1] South Cent Univ Nationalities, Sch Pharmaceut Sci, 182 Min Zu Rd, Wuhan 430074, Hubei, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2017年 / 39卷

基金：

中国国家自然科学基金;

关键词：

Hypoglycemic; beta-ecdysterone; GLUT4; Akt; IRS-1; IR beta; INSULIN-RESISTANCE; GLUCOSE-TRANSPORTER; ANTIDIABETIC ACTIVITY; GLUT4; TRANSLOCATION; MUSCLE; HYPERGLYCEMIA; OBESITY; INFLAMMATION; APOPTOSIS; EXTRACT;

D O I：

10.1016/j.jff.2017.09.061

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Our present study investigated the anti-diabetic activity and potential mechanism of O-ecdysterone (beta-EC) derived from Cyanotis arachnoidea. In vitro, beta-EC exhibited a promising effect on increasing GLUT4 translocation by 1.6 folds and glucose uptake by 1.75 folds in L6 cells. In vivo, KK-Ay mice's body weight, blood glucose levels and other related blood-lipid indexes can be significantly reduced with beta-EC treatment. For the mechanism study, we have found that beta-EC exhibited a significant protective effect on insulin resistance (IR) in L6 and HepG2 cells through increasing expressions of IR beta, p-Akt, p-IRS-1 and increasing the expressions of GLUT4 and GLUT2. The phosphorylation of Akt, IRS-1 and the expression of itzp, GLUT4 and GLUT2 in the liver and skeletal muscle in KK-Ay mice were also significantly ameliorated after 4-weeks treatment with beta-EC. According to our present findings, we could conclude that beta-EC possessed the potential anti-diabetic effects through activating IRS-1/AKT/GLUT4 and IRS-1/AICT/GLUT2 pathways. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：123 / 132

页数：10

共 41 条

[1] Adipose-selective targeting of the GLUT4 gene impairs insulin action in muscle and liver [J].