Molecular structure of the PHA synthesis gene cluster from new mcl-PHA producer Pseudomonas putida KCTC1639

Pseudomonas putida KCTC1639 was newly identified as a potential producer of biodegradable medium chain length polyhydroxyalkanoates. It exhibited a carbon assimilation pattern quite different from other known P. putida strains, but a more similar pattern with P. oleovorans, which assimilates the carbon sources mainly through beta-oxidation rather than the fatty acid biosynthesis pathway. The PHA synthesis gene cluster from P putida KCTC 163 9 was composed of two gene loci; the PHA synthase gene locus and granule-associated gene locus, which were cloned and deposited in the GenBank under accession numbers AY286491 and AY750858 as a new nucleotide sequence, respectively. The molecular structure and amino acid homology of the new gene cluster were compared with those from Pseudomonas species, including other P putida strains and P oleovorans, and a higher than 90% homology was observed.