In vivo imaging-guided microsurgery based on femtosecond laser produced new fluorescent compounds in biological tissues

被引：14
作者：
Sun, Qiqi [1 ]
Qin, Zhongya [1 ]
Wu, Wanjie [1 ]
Lin, Yue [2 ]
Chen, Congping [1 ]
He, Sicong [1 ]
Li, Xuesong [1 ]
Wu, Zhenguo [3 ,4 ,5 ]
Luo, Yi [2 ]
Qu, Jianan Y. [1 ,4 ,5 ]
机构：
[1] Hong Kong Univ Sci & Technol, Dept Elect & Comp Engn, Kowloon, Hong Kong, Peoples R China
[2] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Bio X Div, Hefei, Anhui, Peoples R China
[3] Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China
[4] Hong Kong Univ Sci & Technol, Ctr Syst Biol & Human Hlth, Sch Sci, Kowloon, Hong Kong, Peoples R China
[5] Hong Kong Univ Sci & Technol, Inst Adv Study, Kowloon, Hong Kong, Peoples R China
来源：
BIOMEDICAL OPTICS EXPRESS | 2018年 / 9卷 / 02期
关键词：
MULTIPHOTON MICROSCOPY; NANOSURGERY; ABLATION;
D O I：
10.1364/BOE.9.000581
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Femtosecond laser microsurgery has become an advanced method for clinical procedures and biological research. The tissue treated by femtosecond laser can become highly fluorescent, indicating the formation of new fluorescent compounds that can naturally label the treated tissue site. We systematically characterized the fluorescence signals produced by femtosecond laser ablation in biological tissues in vivo. Our findings showed that they possess unique fluorescence properties and can be clearly differentiated from endogenous signals and major fluorescent proteins. We further demonstrated that the new fluorescent compounds can be used as in vivo labelling agent for biological imaging and guided laser microsurgery. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
引用
收藏
页码：581 / 590
页数：10
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