Exploring the mechanism of high degree of delignification inhibits cellulose conversion efficiency

被引：27

作者：

Ding, Dayong ^{[1
]}

Zhou, Xia ^{[1
]}

You, Tingting ^{[1
]}

Zhang, Xun ^{[1
]}

Zhang, Xueming ^{[1
]}

Xu, Feng ^{[1
]}

机构：

[1] Beijing Forestry Univ, Beijing Key Lab Lignocellulos Chem, Beijing 100083, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2018年 / 181卷

基金：

国家重点研发计划;

关键词：

Delignification; Cellulose conversion; Topochemistry; Morphology; Pore structure; PLANT-CELL WALL; ENZYMATIC-HYDROLYSIS; DILUTE-ACID; LIGNIN; PRETREATMENT; BIOMASS; POPLAR; WOOD; ADSORPTION; REMOVAL;

D O I：

10.1016/j.carbpol.2017.11.057

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study explored the mechanism that high degree of delignification (DD) inhibits enzymatic hydrolysis. Sample with DD of 86.22% achieved the highest cellulose conversion of 68.26%, and the cell wall exhibited defibrillation of macrofibrils and erosion of microfibrils during enzymatic hydrolysis. Cracks between microfibrils are formed within the cell wall, getting the largest specific surface area, which greatly enhanced cellulose conversion. However, high DD of 96.58% resulted in dramatic reduction of cellulose conversion to 56.60% which was evidenced to be the synergistic effect of internal cell wall collapse and microfibrils reaggregation. These ultrastructural changes dominated upon this condition and induced a more compact surface structure which significantly hinders the accessibility of cellulase. The CrI value increased after delignification but changed little with the increased DD, suggesting limited influence of DD on crystalline structure. The results indicate that certain amount of lignin retained may be essential to enhance cellulose conversion.

引用

页码：931 / 938

页数：8

共 40 条

[21] Cellulase digestibility of pretreated biomass is limited by cellulose accessibility [J].

Jeoh, Tina ;

Ishizawa, Claudia I. ;

Davis, Mark F. ;

Himmel, Michael E. ;

Adney, William S. ;

Johnson, David K. .

BIOTECHNOLOGY AND BIOENGINEERING, 2007, 98 (01) :112-122

[22] Tissue specific response of Miscanthus x giganteus to dilute acid pretreatment for enhancing cellulose digestibility [J].

Ji, Zhe ;

Zhang, Xun ;

Ling, Zhe ;

Sun, Run-Cang ;

Xu, Feng .

CARBOHYDRATE POLYMERS, 2016, 154 :247-256

[23] Disparate roles of solvent extractable lignin and residual bulk lignin in enzymatic hydrolysis of pretreated sweetgum [J].

Lai, Chenhuan ;

Tu, Maobing ;

Yong, Qiang ;

Yu, Shiyuan .

RSC ADVANCES, 2015, 5 (119) :97966-97974

[24] A pore-hindered diffusion and reaction model can help explain the importance of pore size distribution in enzymatic hydrolysis of biomass [J].

Luterbacher, Jeremy S. ;

Parlange, Jean-Yves ;

Walker, Larry P. .

BIOTECHNOLOGY AND BIOENGINEERING, 2013, 110 (01) :127-136

[25]

Meng XZ, 2015, GREEN CHEM, V17, P4239, DOI [10.1039/C5GC00689A, 10.1039/c5gc00689a]

[26] Genomics of cellulosic biofuels [J].

Rubin, Edward M. .

NATURE, 2008, 454 (7206) :841-845

[27] The effects of lignin removal and drying on the porosity and enzymatic hydrolysis of sugarcane bagasse [J].

Santi Junior, Celso ;

Ferreira Milagres, Adriane Maria ;

Ferraz, Andre ;

Carvalho, Walter .

CELLULOSE, 2013, 20 (06) :3165-3177

[28] Enhancement of cellulose hydrolysis in sugarcane bagasse by the selective removal of lignin with sodium chlorite [J].

Siqueira, Germano ;

Varnai, Aniko ;

Ferraz, Andre ;

Milagres, Adriane M. F. .

APPLIED ENERGY, 2013, 102 :399-402

[29]

Sluiter A., 2008, DETERMINATION SUGARS

[30] TRICHODERMA-REESEI HAS NO TRUE EXO-CELLULASE - ALL INTACT AND TRUNCATED CELLULASES PRODUCE NEW REDUCING END GROUPS ON CELLULOSE [J].

STAHLBERG, J ;

JOHANSSON, G ;

PETTERSSON, G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1157 (01) :107-113

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