An efficient transient expression system for gene function analysis in rose

被引:33
作者
Lu, Jun [1 ,2 ]
Bai, Mengjuan [1 ,2 ]
Ren, Haoran [1 ,2 ]
Liu, Jinyi [1 ,2 ]
Wang, Changquan [1 ,2 ]
机构
[1] Nanjing Agr Univ, Coll Hort, 1 Weigang, Nanjing 210095, Jiangsu, Peoples R China
[2] Nanjing Agr Univ, Key Lab Landscaping, Minist Agr, Nanjing 210095, Jiangsu, Peoples R China
来源
PLANT METHODS | 2017年 / 13卷
基金
中国博士后科学基金;
关键词
Roses; Agrobacterium; Transient transformation; Shoots in vitro; TFL1; HOMOLOG; ARABIDOPSIS; PROTEIN; TRANSCRIPTION; EXPANSION; REGULATOR; TOMATO; PLANTS; PETALS; ROKSN;
D O I
10.1186/s13007-017-0268-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Background: Roses are widely used as garden ornamental plants and cut flowers. Rosa chinensis cv 'Old Blush' has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformation systems is a handicap limiting functional genetics studies of roses. Agrobacterium-mediated transient transformation offers a powerful tool for the characterization of gene function in plants. Results: A convenient and highly efficient Agrobacterium mediated genetic transformation protocol using R. chinensis cv 'Old Blush' seedlings in vitro as an expression system is described in this paper. The most important factor affecting transformation efficiency in this system is seedling age; 3/4-week-old rose shoots with or without roots from sub-culturing are optimal for transformation, requiring no complicated inoculation media, supplements, or carefully tuned plant growth conditions. This transient expression system was successfully applied to analysis of the gene promoter activities, DNA binding capacity of transcription factors, protein-protein interaction in physiological contexts using luciferase as a reporter gene. Conclusions: This transient transformation system was validated as a robust and efficient platform, thus providing a new option for gene function and signaling pathway investigation in roses and further extending the utility of R. chinensis cv 'Old Blush' as a model plant to study diverse gene function and signaling pathways in Rosaceae.
引用
收藏
页数:12
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