Multicolor luciferase assay system: one-step monitoring of multiple gene expressions with a single substrate

被引:76
作者
Nakajima, Y
Kimura, T
Sugata, K
Enomoto, T
Asakawa, A
Kubota, H
Ikeda, M
Ohmiya, Y [1 ]
机构
[1] Natl Inst Adv Ind Sci & Technol, Res Inst Cell Engn, Ikeda, Osaka 5638577, Japan
[2] Saitama Med Sch, Moroyama, Saitama, Japan
[3] ATTO Corp, Tokyo, Japan
关键词
D O I
10.2144/05386ST03
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression. However the expression of more than one gene cannot be monitored simultaneously by this system because one of the two reporting luciferases must be used as an internal control. We have developed a novel reporter assay, system in which three luciferases that emit green, orange, and red light with a single substrate tire used as reporter genes. The activities of the luciferases can be measured simultaneously and quantitatively with optical filters. This system enables us to simply, and rapidly, monitor multiple gene expressions in a one-step reaction.
引用
收藏
页码:891 / 894
页数:4
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