350-kDa royal jelly glycoprotein (apisin), which stimulates proliferation of human monocytes, bears the β1-3galactosylated N-glycan:: Analysis of the N-glycosylation site

被引：60
作者：
Kimura, M
Kimura, Y
Tsumura, K
Okihara, K
Sugimoto, H
Yamada, H
Yonekura, M
机构：
[1] Okayama Univ, Dept Bioresource Chem, Fac Agr, Okayama 7008530, Japan
[2] Kurashiki Sakuyo Univ, Fac Food Culture, Dept Food Syst, Kurashiki, Okayama 7100292, Japan
[3] Yamada Apiculture Ctr Inc, Kagamino, Okayama 7080393, Japan
[4] Ibaraki Univ, Dept Bioresource Sci, Sch Agr, Ami, Ibaraki 3000393, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2003年 / 67卷 / 09期
关键词：
royal jelly; insect glycoprotein; N-glycosylation; site analysis; Apis mellifera;
D O I：
10.1271/bbb.67.2055
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
While doing a structural analysis of minor component N-glycans linked to 350-kDa royal jelly glycoprotein (RJGP), which stimulates the proliferation of human monocytes, we found that a Galbeta1-3GlcNAcbeta1-4Man unit occurs on the insect glycoprotein. The structure of the fluorescence-labeled N-glycan was analyzed by sugar component analysis, IS-MS, and H-1-NMR. The structural analysis showed that the 350-kDa RJGP bears Galbeta1-3GlcNAcbeta1-4(GlcNAcbeta1-2)Manalpha1-3 (Manalpha1-3Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc, suggesting this insect glycoprotein is one of the substrates for both beta1-3 galactosyl and beta1-4 N-acetylglucosamininyl transferases. To our knowledge, this is the first report that succeeded in identifing an insect glycoprotein bearing the beta1-3 galactosylated N-glycan.
引用
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页码：2055 / 2058
页数：4
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