Development of amphiphilic γ-PGA-nanoparticle based tumor vaccine:: Potential of the nanoparticulate cytosolic protein delivery carrier

被引：57

作者：

Yoshikawa, Tomoaki ^{[1
,2
]}

Okada, Naoki ^{[1
,2
]}

Oda, Atsushi ^{[1
,2
]}

Matsuo, Kazuhiko ^{[1
,2
]}

Matsuo, Keisuke ^{[1
,2
]}

Mukai, Yohei ^{[1
]}

Yoshioka, Yasuo ^{[1
,3
]}

Akagi, Takami ^{[2
,4
]}

Akashi, Mitsuru ^{[2
,3
,4
]}

Nakagawa, Shinsaku ^{[1
,2
,3
]}

机构：

[1] Osaka Univ, Dept Biotechnol & Therapeut, Grad Sch Pharmaceut Sci, Suita, Osaka 5650871, Japan

[2] Japan Sci & Technol Agcy, CREST, Tokyo, Japan

[3] Osaka Univ, Ctr Adv Med Engn & Informat, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Grad Sch Engn, Dept Appl Chem, Suita, Osaka 5650871, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 366卷 / 02期

关键词：

nanoparticle; tumor vaccine; antigen presentation/processing; antigen-presenting cells;

D O I：

10.1016/j.bbrc.2007.11.153

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanoscopic therapeutic systems that incorporate biomacromolecules, such as protein and peptides, are emerging as the next generation of nanomedicine aimed at improving the therapeutic efficacy of biomacromolecular drugs. In this study, we report that poly(gamma-glutamic acid)-based nanoparticles (gamma-PGA NPs) are excellent protein delivery carriers for tumor vaccines that delivered antigenic proteins to antigen-presenting cells and elicited potent immune responses. Importantly, gamma-PGA NPs efficiently delivered entrapped antigenic proteins through cytosolic translocation from the endosomes, which is a key process of gamma-PGA NP-mediated anti-tumor immune responses. Our findings suggest that the gamma-PGA NP system is suitable for the intracellular delivery of protein-based drugs as well as tumor vaccines. (C) 2007 Published by Elsevier Inc.

引用

页码：408 / 413

页数：6

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