Effects of Growth Phase and Cell Density on Cryptogein-induced Programmed Cell Death in Suspension-cultured Tobacco BY-2 Cells: Development of a Model System for 100% Efficient Hypersensitive Cell Death

被引：2

作者：

Sawai, Yu ^{[1
]}

Tamotsu, Satoshi ^{[1
]}

Kuchitsu, Kazuyuki ^{[2
]}

Sakai, Atsushi ^{[3
]}

机构：

[1] Nara Womens Univ, Grad Sch Humanities & Sci, Nara 6308506, Japan

[2] Tokyo Univ Sci, Dept Appl Biol Sci, Chiba 2788510, Japan

[3] Nara Womens Univ, Fac Sci, Dept Biol Sci, Nara 6308506, Japan

来源：

CYTOLOGIA | 2010年 / 75卷 / 04期

关键词：

Elicitor; Cryptogein; Hypersensitive reaction; HR cell death; Tobacco BY-2 cells; PHYTOPHTHORA-CRYPTOGEA; PROLONGED ACTIVATION; SIGNAL-TRANSDUCTION; OXIDATIVE BURST; SALICYLIC-ACID; ELICITOR; INDUCTION; CYCLE; DNA; ACCUMULATION;

D O I：

10.1508/cytologia.75.389

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We developed a novel model system for hypersensitive cell death, in which the elicitor induced cell death in suspension-cultured cells with 100% efficiency. The 100% efficient cell death was achieved when tobacco BY-2 cells at stationary-phase were diluted to 1 X 10(5) cells/ml with conditioned medium and treated with 1.5 mu M of cryptogein, an oomycete-derived proteinaceous elicitor. During the cell-death process, nuclear chromatin fragmentation, activation of several genes associated with hypersensitive response (HR), and induction of several genes related to antimicrobial activity were observed. The results strongly suggest that the cells undergoing HR cell death activated antimicrobial mechanism while executing cell-death program.

引用

页码：389 / 396

页数：8

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