Immunohistochemical distribution of leptin in kidney tissues of melatonin treated diabetic rats

被引：8

作者：

Yildiz, S. Elis ^{[1
]}

Deprem, T. ^{[2
]}

Sari, E. Karadag ^{[2
]}

Bingol, S. A. ^{[1
]}

Tasci, S. Koral ^{[2
]}

Aslan, S. ^{[2
]}

Nur, G. ^{[3
]}

Sozmen, M. ^{[4
]}

机构：

[1] Kafkas Univ, Sch Hlth Sci, Kars, Turkey

[2] Kafkas Univ, Fac Vet Med, Dept Histol & Embryol, Kars, Turkey

[3] Directorate Food Control Lab, Antakya, Turkey

[4] Ondokuz Mayis Univ, Dept Pathol, Fac Vet Med, Samsun, Turkey

来源：

BIOTECHNIC & HISTOCHEMISTRY | 2015年 / 90卷 / 04期

关键词：

diabetes mellitus; immunohistochemistry; kidney; leptin; melatonin; GENE-EXPRESSION PROFILE; MELLITUS; OBESITY; MICE; DEFICIENCY;

D O I：

10.3109/10520295.2014.983548

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We examined using immunohistochemistry the distribution of leptin in kidney tissues of melatonin treated, streptozotocin (STZ) diabetic rats. The animals were divided into five groups: control, sham, melatonin-treated, diabetic and melatonin-treated diabetic. Kidney sections were prepared and stained with hematoxylin and eosin, and Crossman's triple staining for histological examination. The immunohistochemical localization of leptin in the kidney tissue was determined using the streptavidin-biotin-peroxidase method. We determined that on days 7 and 14, the leptin immunoreactivity of the diabetic and melatonin-treated diabetic groups was weaker than for the other groups. Weak immunoreactivity was found in the proximal and distal tubules of the kidney in the diabetic and melatonin-treated diabetic groups on days 7 and 14, and strong immunoreactivity was found in the control, sham and melatonin groups. Melatonin application had no significant effect on leptin production in the kidney tissues of diabetic rats.

引用

页码：270 / 277

页数：8

共 37 条

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