Impedimetric aptasensor for tobramycin detection in human serum

被引：42

作者：

Gonzalez-Fernandez, Eva ^{[1
]}

de-los-Santos-Alvarez, Noemi ^{[1
]}

Jesus Lobo-Castanon, Maria ^{[1
]}

Jose Miranda-Ordieres, Arturo ^{[1
]}

Tunon-Blanco, Paulino ^{[1
]}

机构：

[1] Univ Oviedo, Dpto Quim Fis & Analit, E-33006 Oviedo, Spain

来源：

BIOSENSORS & BIOELECTRONICS | 2011年 / 26卷 / 05期

关键词：

Aptamer; Modified-RNA; Faradaic impedance spectroscopy; Antibiotic; Biosensor; SACCHARIDE-RNA RECOGNITION; TANDEM-MASS-SPECTROMETRY; AMINOGLYCOSIDE ANTIBIOTICS; CAPILLARY-ELECTROPHORESIS; LIQUID-CHROMATOGRAPHY; NEOMYCIN-B; APTAMERS; ASSAY; DERIVATIZATION; VALIDATION;

D O I：

10.1016/j.bios.2010.10.011

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An RNA aptamer is proposed as a recognition element for the detection of tobramycin in human serum. A displacement assay was developed using faradaic-electrochemical impedance spectroscopy (F-EIS) as a detection technique. Two modified aptamers, a partially (ATA) and a fully O-methylated aptamer (FATA) were evaluated and compared. The affinity constant, K-D), for both aptamers was estimated by F-EIS resulting virtually identical within the experimental error. The selectivity towards other aminoglycosides was also studied. The analytical characteristics were evaluated in aqueous solution using both aptamers and FATA was selected for human serum experiments. Using a 1:0.5 dilution of the serum, a linear range between 3 mu M and 72.1 mu M was obtained, which included the therapeutic range of the antibiotic. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：2354 / 2360

页数：7

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